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AB251257

Anti-LIM2 antibody [EPR16947] - BSA and Azide free

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Rabbit Recombinant Monoclonal LIM2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

Lens fiber membrane intrinsic protein, MP18, MP19, MP20, LIM2

2 Images
Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)
  • WB

Lab

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

This data was developed using ab198502, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LIM2 antibody [EPR16947] (<a href='/en-us/products/primary-antibodies/lim2-antibody-epr16947-ab198502'>ab198502</a>) at 1/50000 dilution

Lane 1:

Mouse eyeball tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Lane 4:

Mouse kidney tissue lysate at 10 µg

Lane 5:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

true

Exposure time: 30s

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)
  • WB

Lab

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

This data was developed using ab198502, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LIM2 antibody [EPR16947] (<a href='/en-us/products/primary-antibodies/lim2-antibody-epr16947-ab198502'>ab198502</a>) at 1/5000 dilution

Lane 1:

Rat eyeball tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Lane 4:

Rat kidney tissue lysate at 10 µg

Lane 5:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16947

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251257 is the carrier-free version of ab198502.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LIM2 protein also known as lens intrinsic membrane protein 2 is a lens-specific protein with a molecular mass of approximately 22 kDa. It is part of the eye lens fiber cells and is critical in maintaining lens transparency and structural integrity. LIM2 is highly expressed in the lens contributing to its function in the eye.
Biological function summary

LIM2 plays a significant role in lens development and homeostasis. It is part of the integral membrane proteins that influence lens fiber cell maturation and intercellular communication. LIM2 interacts with other cellular components supporting the structural arrangement necessary for proper lens function.

Pathways

LIM2 participates in cell adhesion and signal transduction pathways in the eye lens. These pathways are essential for maintaining cell shape transparency and communication within lens cells. LIM2 functions in concert with proteins like connexins which are also integral to cell-cell communication within the lens.

Mutations in the LIM2 gene have been linked to cataracts and other lens-related abnormalities. These conditions often result from disruptions in lens transparency and refractive properties that LIM2 helps maintain. The alpha crystallin and gamma crystallin proteins are related to LIM2 as their interactions affect lens clarity and structure contributing to cataract formation when dysfunctional.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.
See full target information LIM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com