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AB96178

Anti-LIMCH1 antibody

5

(2 Reviews)

|

(7 Publications)

Rabbit Polyclonal LIMCH1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIMCH1 aa 700 to C-terminus.

View Alternative Names

KIAA1102, LIMCH1, LIM and calponin homology domains-containing protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMCH1 antibody (AB96178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMCH1 antibody (AB96178)

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab96178 antibody at 1 : 500 dilution.

Western blot - Anti-LIMCH1 antibody (AB96178)
  • WB

Supplier Data

Western blot - Anti-LIMCH1 antibody (AB96178)

Run on 5% SDS PAGE gel

All lanes:

Western blot - Anti-LIMCH1 antibody (ab96178) at 1/2000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 122 kDa

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Western blot - Anti-LIMCH1 antibody (AB96178)
  • WB

Unknown

Western blot - Anti-LIMCH1 antibody (AB96178)

All lanes:

Western blot - Anti-LIMCH1 antibody (ab96178) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 122 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LIMCH1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggest antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LIM and Calponin Homology Domain 1 known as LIMCH1 is a protein with a mass of approximately 90 kDa. It functions mechanically by interacting with actin filaments influencing their organization and stability. LIMCH1 contains a LIM domain which provides a scaffold for protein-protein interactions and a calponin homology domain which binds actin. LIMCH1 is expressed in various tissues including skeletal muscle heart and brain indicating its involvement in multiple cellular functions.
Biological function summary

LIMCH1 modulates cytoskeletal dynamics and plays a role in cell shape and motility. It forms part of protein complexes that regulate the architecture of the actin cytoskeleton. These complexes involve other actin-binding proteins contributing to processes like cell adhesion and migration. LIMCH1 influences the remodeling of cytoskeletal structures critical for cellular responses to environmental changes and mechanical stress.

Pathways

Researchers identify LIMCH1 as a component in the RhoA signaling pathway which controls actin cytoskeleton dynamics. LIMCH1 interacts with proteins like Rho-associated protein kinase (ROCK) and myosin light chain kinase (MLCK) coordinating actomyosin contractility. Another significant pathway involving LIMCH1 is the regulation of focal adhesion where it associates with vinculin and talin contributing to the linkage between the plasma membrane and the cytoskeletal framework.

Abnormalities in LIMCH1 expression or function relate to conditions such as heart hypertrophy and certain types of cancer. In cardiac muscle cells disrupted LIMCH1 activity can lead to altered contractility linking it to heart disease. Furthermore in the context of cancer LIMCH1's modulation of cell migration and adhesion connects it to metastasis. Studies also find its interactions with proteins like p53 a well-known tumor suppressor as important in these disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.
See full target information LIMCH1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:419 PubMed41063153

2025

LIMCH1 inhibits antitumor immunity by upregulating PDL1 in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minghan Qiu,Zhanhua Gao,Mengran Tian,Yuya Liu,Jinpu Liu,Qiaonan Zhang,Ruxue Liu,Yayun Wang,Xiangqian Zheng,Jie Hao,Zhen Yang,Ming Gao

Scientific reports 15:1446 PubMed39789044

2025

Integrative bioinformatics and immunohistochemical analysis unravel the prognostic significance and immunological implication of LIMCH1 in breast cancer: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Bei Li,Wenge Li,Jingping Yuan,Shengrong Sun,Juanjuan Li

The European respiratory journal 65: PubMed39401861

2024

Evidence for a lipofibroblast-to- myofibroblast reversible switch during the development and resolution of lung fibrosis in young mice.

Applications

Unspecified application

Species

Unspecified reactive species

Arun Lingampally,Marin Truchi,Olivier Mauduit,Vanessa Delcroix,Esmeralda Vasquez-Pacheco,Marine Gautier-Isola,Xuran Chu,Ali Khadim,Cho-Ming Chao,Mahsa Zabihi,Sara Taghizadeh,Stefano Rivetti,Manuela Marega,Alena Moiseenko,Stefan Hadzic,Ana Ivonne Vazquez-Armendariz,Susanne Herold,Stefan Günther,Pamela Millar-Büchner,Janine Koepke,Christos Samakovlis,Jochen Wilhelm,Marek Bartkuhn,Thomas Braun,Norbert Weissmann,JinSan Zhang,Malgorzata Wygrecka,Helen P Makarenkova,Andreas Günther,Werner Seeger,Chengshui Chen,Elie El Agha,Bernard Mari,Saverio Bellusci

Thoracic cancer 12:1959-1972 PubMed34096174

2021

Circular RNA circLDB2 functions as a competing endogenous RNA to suppress development and promote cisplatin sensitivity in non-squamous non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Luguang Li,Weiyu Zhang,Guojun Zhang

Nature communications 12:262 PubMed33431859

2021

Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Samaneh K Sarvestani,Steven Signs,Bo Hu,Yunku Yeu,Hao Feng,Ying Ni,David R Hill,Robert C Fisher,Sylvain Ferrandon,Reece K DeHaan,Jennifer Stiene,Michael Cruise,Tae Hyun Hwang,Xiling Shen,Jason R Spence,Emina H Huang

Journal of Cancer 12:181-189 PubMed33391414

2021

Loss of LIMCH1 predicts poor prognosis in patients with surgically resected Lung Adenocarcinoma: A study based on Immunohistochemical Analysis and Bioinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

He Cao,Jing Zhao,Zhen Chen,Wenjia Sun,Kexin Ruan,Jianya Zhou,Jianying Zhou

Biochimica et biophysica acta. Molecular basis of 1863:1596-1604 PubMed28213125

2017

Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Terry Jo V Bichell,Michal Wegrzynowicz,K Grace Tipps,Emma M Bradley,Michael A Uhouse,Miles Bryan,Kyle Horning,Nicole Fisher,Karrie Dudek,Timothy Halbesma,Preethi Umashanker,Andrew D Stubbs,Hunter K Holt,Gunnar F Kwakye,Andrew M Tidball,Roger J Colbran,Michael Aschner,M Diana Neely,Alba Di Pardo,Vittorio Maglione,Alexander Osmand,Aaron B Bowman
View all publications
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