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AB114104

Anti-LIMD1 antibody

2

(1 Review)

|

(1 Publication)

Rabbit Polyclonal LIMD1 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LIMD1 aa 1-50.

View Alternative Names

LIM domain-containing protein 1, LIMD1

1 Images
Immunoprecipitation - Anti-LIMD1 antibody (AB114104)
  • IP

Unknown

Immunoprecipitation - Anti-LIMD1 antibody (AB114104)

Detection of Human LIMD1 by Western blot of Immunoprecipitate.
LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 μg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 μg/ml.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-LIMD1 antibody (ab114104)

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human LIMD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab114104 was affinity purified using an epitope specific to LIMD1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMD1 also known as LIM domain-containing protein 1 is a protein with a molecular mass of approximately 76 kDa. It acts mechanically by functioning as a scaffolding protein important for assembling protein complexes at the cellular membrane. LIMD1 is expressed in many tissues with higher levels in lung and heart tissues. It contains LIM domains which enable protein-protein interactions necessary for proper cellular functions.
Biological function summary

LIM domain-containing protein 1 interacts with various proteins to regulate important cellular processes including cell proliferation and adhesion. It is part of the ubiquitin ligase complex and contributes to the regulation of gene expression by modulating transcriptional activity. Its function in gene silencing shows its role in maintaining normal cellular physiology and alterations can contribute to disease states.

Pathways

LIMD1 is involved in the Hippo signaling pathway and plays a role in cell contact inhibition and organ size control. It interacts with proteins like YAP (Yes-associated protein) to help control these processes. In addition LIMD1 contributes to the regulation of the p53 pathway inhibiting tumor progression by supporting p53-mediated cell cycle arrest and apoptosis.

LIMD1 plays important roles in cancer and respiratory diseases. Its interaction with p53 links it to tumor suppression where a loss or dysfunction of LIMD1 is often seen in cancers like lung cancer. Also its role in respiratory health associates it with COPD where alterations of LIMD1 may exacerbate disease progression. The relationship between LIMD1 and these diseases emphasizes its potential utility as a therapeutic target or biomarker.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.
See full target information LIMD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:15439-15454 PubMed30082317

2018

The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Rodríguez-Castañeda,Roza Berhanu Lemma,Ignacio Cuervo,Mads Bengtsen,Lisa Marie Moen,Marit Ledsaak,Ragnhild Eskeland,Odd Stokke Gabrielsen
View all publications
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Product promise

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