AB225714

Anti-LIMD1 antibody

Name: Anti-LIMD1 antibody (ab225714) | Abcam
Brand: Abcam Limited
SKU: AB225714
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal LIMD1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LIMD1 aa 50-100.

View Alternative Names

LIM domain-containing protein 1, LIMD1

2 Images

Supplier Data

Immunoprecipitation - Anti-LIMD1 antibody (AB225714)

LIMD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225714 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225714 at 0.4 μg/ml.

Lane 1 : ab225714 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-LIMD1 antibody (ab225714)

Predicted band size: 72 kDa

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Supplier Data

Western blot - Anti-LIMD1 antibody (AB225714)

All lanes:

Western blot - Anti-LIMD1 antibody (ab225714) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 72 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human LIMD1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UGP4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMD1 also known as LIM domain-containing protein 1 is a protein with a molecular mass of approximately 76 kDa. It acts mechanically by functioning as a scaffolding protein important for assembling protein complexes at the cellular membrane. LIMD1 is expressed in many tissues with higher levels in lung and heart tissues. It contains LIM domains which enable protein-protein interactions necessary for proper cellular functions.

Biological function summary

LIM domain-containing protein 1 interacts with various proteins to regulate important cellular processes including cell proliferation and adhesion. It is part of the ubiquitin ligase complex and contributes to the regulation of gene expression by modulating transcriptional activity. Its function in gene silencing shows its role in maintaining normal cellular physiology and alterations can contribute to disease states.

Pathways

LIMD1 is involved in the Hippo signaling pathway and plays a role in cell contact inhibition and organ size control. It interacts with proteins like YAP (Yes-associated protein) to help control these processes. In addition LIMD1 contributes to the regulation of the p53 pathway inhibiting tumor progression by supporting p53-mediated cell cycle arrest and apoptosis.

LIMD1 plays important roles in cancer and respiratory diseases. Its interaction with p53 links it to tumor suppression where a loss or dysfunction of LIMD1 is often seen in cancers like lung cancer. Also its role in respiratory health associates it with COPD where alterations of LIMD1 may exacerbate disease progression. The relationship between LIMD1 and these diseases emphasizes its potential utility as a therapeutic target or biomarker.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.

See full target information LIMD1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIMD1 antibody

Immunoprecipitation - Anti-LIMD1 antibody (AB225714)

Western blot - Anti-LIMD1 antibody (AB225714)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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