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AB188871

Anti-LIME antibody [EPR14713]

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(1 Publication)

Rabbit Recombinant Monoclonal LIME antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LIME, LP8067, LIME1, Lck-interacting transmembrane adapter 1, Lck-interacting membrane protein, Lck-interacting molecule

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (AB188871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (AB188871)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (AB188871)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (AB188871)

Immunohistochemical analysis of paraffin-embedded Human mammary gland infiltrating duct carcinoma tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-LIME antibody [EPR14713] (AB188871)
  • WB

Supplier Data

Western blot - Anti-LIME antibody [EPR14713] (AB188871)

All lanes:

Western blot - Anti-LIME antibody [EPR14713] (ab188871) at 1/1000 dilution

All lanes:

Human thymus lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 31 kDa

false

Western blot - Anti-LIME antibody [EPR14713] (AB188871)
  • WB

Supplier Data

Western blot - Anti-LIME antibody [EPR14713] (AB188871)

All lanes:

Western blot - Anti-LIME antibody [EPR14713] (ab188871) at 1/10000 dilution

All lanes:

HuT-78 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa

false

  • Carrier free

    Anti-LIME antibody [EPR14713] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14713

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIME also known as Lck-interacting molecule is a transmembrane adaptor protein with a molecular weight of approximately 30 kDa. LIME is expressed mainly in immune cells such as T cells B cells and NK cells playing a significant role in immune system signaling. Its structural features include several tyrosine-based signaling motifs that interact with lymphocyte-specific protein tyrosine kinases. These interactions support LIME's function as a mediator in signaling cascades within immune cells.
Biological function summary

The LIME protein functions by helping to organize and transduce signals involved in lymphocyte activation proliferation and differentiation. It serves as a scaffold that aggregates proteins involved in signaling pathways forming part of larger signal transduction complexes. LIME directly associates with Src family kinases influencing cell signaling related to the immune response. This characteristic enables it to modulate various intracellular processes especially those concerning leukocytes and their responses to external stimuli.

Pathways

LIME participates in the T-cell receptor (TCR) signaling pathway and B-cell receptor (BCR) signaling pathway both important for adaptive immunity. In the TCR signaling pathway LIME interacts with ZAP-70 protein aiding T-cell activation and response to antigens. In the BCR signaling pathway LIME associates with kinases to modulate B-cell responses. Through these interactions LIME plays a role in optimizing the immune system's function and its ability to respond to pathogens or altered cells.

LIME has been linked to immune-related conditions like autoimmunity and lymphoma. Aberrant expression or mutations in LIME can disrupt normal signal transduction potentially leading to autoimmune diseases due to improper immune cell activation. Similarly altered LIME signaling has associations with lymphomas where it may impact cell proliferation and survival pathways. Changes in LIME function could affect its interacting partners such as Src family kinases contributing to the dysregulated growth and survival of immune cells seen in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
See full target information LIME1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33945503

2021

C2orf69 mutations disrupt mitochondrial function and cause a multisystem human disorder with recurring autoinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Lausberg,Sebastian Gießelmann,Joseph P Dewulf,Elsa Wiame,Anja Holz,Ramona Salvarinova,Clara D van Karnebeek,Patricia Klemm,Kim Ohl,Michael Mull,Till Braunschweig,Joachim Weis,Clemens J Sommer,Stephanie Demuth,Claudia Haase,Claudia Stollbrink-Peschgens,François-Guillaume Debray,Cecile Libioulle,Daniela Choukair,Prasad T Oommen,Arndt Borkhardt,Harald Surowy,Dagmar Wieczorek,Norbert Wagner,Robert Meyer,Thomas Eggermann,Matthias Begemann,Emile Van Schaftingen,Martin Häusler,Klaus Tenbrock,Lambert van den Heuvel,Miriam Elbracht,Ingo Kurth,Florian Kraft
View all publications
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Product promise

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