Anti-LIMK2 antibody [EP969Y] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - BSA and Azide free (AB236007)

This data was developed using ab45165, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium cancer tissue labeling LIMK2 with ab45165 at 1/250 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• WB

Lab

Western blot - Anti-LIMK2 antibody [EP969Y] - BSA and Azide free (AB236007)

This data was developed using ab45165, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EP969Y] to LIMK2 ab45165 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 72 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in LIMK2 knockout U-87 MG cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (<a href='/en-us/products/primary-antibodies/limk2-antibody-ep969y-c-terminal-ab45165'>ab45165</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

LIMK2 knockout U-87 MG cell line at 20 µg

Lane 3:

HCT 116 at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 72 kDa

false