Rabbit Polyclonal LIMK2 phospho T505 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human LIMK2 phospho T505.
Images
Publications
Filter by
- Cell communication and signaling : CCS 21:1632023Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiqi Zeng,Zhizhao Deng,Yingxin Gao,Guoliang Sun,Xianlong Li,Dongdong YuanPubMed 37381027
- Life sciences 274:1192262021O-linked N-acetylglucosaminyltransferase OGT inhibits diabetic nephropathy by stabilizing histone methyltransferases EZH2 via the HES1/PTEN axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Fang,Ping LiPubMed 33609540
- Frontiers in pharmacology 11:5204862020Circular RNA Involved in the Protective Effect of L. on Myocardial Ischemic/Re-Perfused Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongzhi Xiao et. al.PubMed 33101012
- Aging 12:2333-23462020Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulei Gu et. al.PubMed 32019904
- Journal of cellular biochemistry 120:19858-198672019Long noncoding RNA Alu-mediated p21 transcriptional regulator promotes proliferation, migration, and pipe-formation of human microvascular endothelial cells by sponging miR-126.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiping Song et. al.PubMed 31310378
- Experimental and molecular pathology 110:1042812019Angelica polysaccharide alleviates oxidative response damage in HaCaT cells through up-regulation of miR-126.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXijun Zhang et. al.PubMed 31288012
- Cell death & disease 10:2612019Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Ren et. al.PubMed 30886151
- Journal of cellular physiology 234:13525-135332019MicroRNA-493-5p inhibits proliferation and metastasis of osteosarcoma cells by targeting Kruppel-like factor 5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaiqing Zhang et. al.PubMed 30773645
- Biochimica et biophysica acta. Molecular cell research 1866:418-4292018Cofilin-1 signaling mediates epithelial-mesenchymal transition by promoting actin cytoskeleton reorganization and cell-cell adhesion regulation in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnie Cristhine Moraes Sousa-Squiavinato,Murilo Ramos Rocha,Pedro Barcellos-de-Souza,Waldemir Fernandes de Souza,Jose Andres Morgado-DiazPubMed 30296500
- Oncology letters 15:3937-39432018miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaokui Xia et. al.PubMed 29456742
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LIMK2 phospho T505. The exact immunogen used to generate this antibody is proprietary information. Database link P53671
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
Alternative names
LIM domain kinase 2, LIMK-2, LIMK2
Recommended products
Rabbit Polyclonal LIMK2 phospho T505 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human LIMK2 phospho T505.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LIMK2 phospho T505. The exact immunogen used to generate this antibody is proprietary information. Database link P53671
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is specific for LIMK2 only when phosphorylated at threonine 505, but may also cross react with LIMK1 phosphorylated at tyrosine 505 based on homology.
- Concentration
- Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LIMK2 also known as LIM domain kinase 2 is a protein that functions primarily as a serine/threonine kinase. This protein with a molecular weight of approximately 72 kDa plays an important role in regulating the cytoskeleton dynamics by phosphorylating and inactivating cofilin thereby affecting actin filament stability. LIMK2 is ubiquitously expressed with higher expression levels observed in the brain and certain types of cancer tissues indicating its importance in both normal and pathological cell processes.
- Biological function summary
LIMK2 influences cell motility differentiation and division by modulating the actin cytoskeleton. It is a central component in various signaling complexes where it interacts with proteins such as ROCK and PAK which are upstream activators. By controlling actin filament assembly and disassembly LIMK2 impacts essential cellular functions including migration and invasion highlighting its potential role in cancer metastasis.
- Pathways
LIMK2 integrates into signaling cascades linked to the Rho family of GTPases and the TGF-beta pathways. In these pathways it collaborates with proteins like ROCK to influence cytoskeletal reorganization. LIMK2's activity in these pathways emphasizes its role in maintaining cellular integrity and responding to extracellular stimuli which are critical components of cell cycle regulation and morphological changes.
- Associated diseases and disorders
Aberrant LIMK2 activity has connections with cancer progression and neurological disorders. In cancer upregulation can lead to increased tumor cell invasion and metastasis through its interaction with cofilin and the subsequent increase in actin filament assembly. In neurological contexts altered LIMK2 function associates with neurodegenerative diseases possibly due to its role in neuronal signaling networks and cytoskeletal dynamics. Understanding LIMK2’s interactions with proteins like cofilin offers insight into potential therapeutic targets for managing these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-LIMK2 (phospho T505) antibody (ab38499)
All lanes: Western blot - Anti-LIMK2 (phospho T505) antibody (ab38499) at 1/500 dilution
Lane 1: MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
Lane 2: MCF7 cell extract (5-30ug of total protein).
SecondaryAll lanes: Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size: 72 kDa
Observed band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 (phospho T505) antibody (ab38499)
ab38499 at a 1:50 dilution staining LIMK2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 (phospho T505) antibody (ab38499)
ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com