AB156699

Anti-LIMKAIN B1 antibody

Name: Anti-LIMKAIN B1 antibody (ab156699) | Abcam
Brand: Abcam Limited
SKU: AB156699
Price: 520 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal LIMKAIN B1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MARF1 aa 1600-1700.

View Alternative Names

KIAA0430, LKAP, MARF1, Meiosis regulator and mRNA stability factor 1, Limkain-b1, Meiosis arrest female protein 1

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Immunoprecipitation - Anti-LIMKAIN B1 antibody (AB156699)

Detection of LIMKAIN B1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab156699 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab156699 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

Lane 1:

Alternative Anti-LIMKAIN B1 antibody

Lane 2:

Immunoprecipitation - Anti-LIMKAIN B1 antibody (ab156699) at 1 µg/mL

Lane 3:

Control IgG

All lanes:

Jurkat whole cell lysate

Predicted band size: 193 kDa

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Western blot - Anti-LIMKAIN B1 antibody (AB156699)

All lanes:

Western blot - Anti-LIMKAIN B1 antibody (ab156699) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 193 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MARF1 aa 1600-1700. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4F3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab156699 was affinity purified using an epitope specific to LIMKAIN B1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMKAIN B1 also called LAS1L is a protein with a mass of approximately 35 kDa. The protein plays a role in ribosome biogenesis and is an essential factor in the nucleoplasmic assembly of pre-ribosomal particles. LIMKAIN B1 is mainly expressed in the nucleus of the cell where it associates with other ribosomal proteins and RNAs to ensure proper ribosome function and production.

Biological function summary

LIMKAIN B1 affects the production and assembly of ribosome components by associating with the Las1L complex. The protein’s activity is required for the processing of 28S rRNA highlighting its importance in ribosome maturation. This processing step happens in the final stage of ribosome biogenesis and disruptions in LIMKAIN B1 function can lead to the accumulation of unprocessed ribosomal RNA impairing the formation of functional ribosomal units.

Pathways

LIMKAIN B1 has significant roles in the ribosome biogenesis and cell proliferation pathways. The protein monitors rRNA processing and ribosome assembly within the nucleolus. LIMKAIN B1 works closely with NOL9 a protein involved in 28S rRNA cleavage to regulate the synthesis of ribosomes. Such coordination ensures that ribosome production matches the overall cellular demand for protein synthesis.

Mutations in LIMKAIN B1 are linked to certain types of cancer and ribosomopathies. Alterations in its function can lead to defective ribosome production contributing to the pathogenesis of ribosomopathies. In the context of cancer LIMKAIN B1 influences the expression of oncoproteins through its relation to Myc a protein that drives cell cycle progression and growth. This association highlights its potential impact on both cancer development and the regulation of cellular growth processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Essential regulator of oogenesis required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Probably acts via some RNA metabolic process, equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Also required to protect from DNA double-strand breaks (By similarity).

See full target information MARF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769053

2021

and Partially Suppress G1 Phase Arrest Caused by Spliceostatin A Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Kikuchi,Daisuke Kaida

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LIMKAIN B1 antibody

Immunoprecipitation - Anti-LIMKAIN B1 antibody (AB156699)

Western blot - Anti-LIMKAIN B1 antibody (AB156699)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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