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Rabbit Recombinant Monoclonal LIMPII antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (AB240186), expandable thumbnail
  • Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (AB240186), expandable thumbnail
  • Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (AB240186), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (AB240186), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (AB240186), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

8 products for Alternative Version

Target data

Function

Acts as a lysosomal receptor for glucosylceramidase (GBA1) targeting. (Microbial infection) Acts as a receptor for enterovirus 71.

Alternative names

CD36, CD36L2, LIMP2, LIMPII, SCARB2, Lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein, CD36 antigen-like 2, Lysosome membrane protein II, Scavenger receptor class B member 2, LGP85, LIMP II

Recommended products

Rabbit Recombinant Monoclonal LIMPII antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12080
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240186 is the carrier-free version of Anti-LIMPII antibody [EPR12080] ab176317.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LIMPII also known as LIMP-2 is a lysosomal membrane protein involved in the transport and degradation processes within cells. It has an approximate molecular mass of 54 kDa. LIMPII is mainly expressed in the lysosomal membranes of a variety of tissues notably in the kidney liver and spleen. Its structural characteristics enable it to function effectively within these cellular organelles delivering enzymes and other molecules essential for cellular maintenance and function.

Biological function summary

The protein plays a critical role in lipid and protein degradation and helps maintain cellular homeostasis. As part of the lysosomal membrane LIMPII facilitates the uptake and processing of large biomolecules into the lysosome. It functions as a receptor for the uptake of β-glucocerebrosidase forming a complex to assist in its proper delivery from the endoplasmic reticulum to the lysosomes. This receptor-ligand relationship ensures efficient enzymatic activity necessary for lipid metabolism.

Pathways

One finds that LIMPII participates in the sphingolipid metabolism pathway and is vital for glucosylceramide catabolism. It interacts with glucocerebrosidase in this pathway which plays a significant role in the metabolism of glycosphingolipids. Another pathway where LIMPII has importance is the autophagy pathway where it contributes to the fusion of autophagosomes with lysosomes. Its activity supports the turnover of cellular components maintaining cellular metabolic balance.

Associated diseases and disorders

LIMPII's dysfunction has links to Gaucher's disease and atherosclerosis. Both conditions relate to improper lipid metabolism. In Gaucher's disease the interaction between LIMPII and glucocerebrosidase becomes disrupted leading to lysosomal storage issues. In contrast altered expressions of LIMPII correlate with atherosclerosis possibly due to its role in lipid processing. These associations suggest that LIMPII could be a significant factor in therapeutic approaches for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Immunoprecipitation - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using Anti-LIMPII antibody [EPR12080] ab176317 at a 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LIMPII antibody [EPR12080] ab176317).

    All lanes: Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (Anti-LIMPII antibody [EPR12080] ab176317)

    Predicted band size: 54 kDa

  • Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    All lanes: Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate

    Lane 2: Y79 (Human retinoblastoma cell line) lysate

    Lane 3: Rat eyeball lysate

    Lane 4: Rat muscle lysate

    Lane 5: Mouse liver lysate

    Predicted band size: 54 kDa

  • Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Western blot - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    False colour image of Western blot: Anti-LIMPII antibody [EPR12080] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-LIMPII antibody [EPR12080] ab176317 was shown to bind specifically to LIMPII. A band was observed at 80 kDa in wild-type MCF7 cell lysates with no signal observed at this size in SCARB2 knockout cell line Human SCARB2 knockout MCF7 cell line ab274952 (knockout cell lysate Human SCARB2 knockout MCF7 cell lysate ab275010). To generate this image, wild-type and SCARB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-LIMPII antibody [EPR12080] (Anti-LIMPII antibody [EPR12080] ab176317) at 1/1000 dilution

    Lane 1: Wild-type MCF7 cell lysate at 20 µg

    Lane 2: SCARB2 knockout MCF7 cell lysate at 20 µg

    Lane 2: Western blot - Human SCARB2 knockout MCF7 cell line (Human SCARB2 knockout MCF7 cell line ab274952)

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 54 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using Anti-LIMPII antibody [EPR12080] ab176317 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LIMPII antibody [EPR12080] ab176317).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using Anti-LIMPII antibody [EPR12080] ab176317 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LIMPII antibody [EPR12080] ab176317).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LIMPII antibody [EPR12080] - BSA and Azide free (ab240186)

    Intracellular flow cytometric analysis of permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling LIMPII using Anti-LIMPII antibody [EPR12080] ab176317 at a 1/10 dilution (red)or a rabbit IgG control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LIMPII antibody [EPR12080] ab176317).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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