Anti-Lin28A antibody [6D1F9]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [6D1F9] (AB76369)

ab76369 at 1/2000 dillution staining Lin28A in NTERA-2 cells by Immunocytochemistry/ Immunofluorescence. The green color in image show positive staining with primary antibody while nuclei were stained blue with DRAQ5 fluorescent DNA dye.

• Flow Cyt

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Flow Cytometry - Anti-Lin28A antibody [6D1F9] (AB76369)

Overlay histogram showing HeLa cells stained with ab76369 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76369, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [6D1F9] (AB76369)

Immunofluorescence staining of methanol fixed HeLa cells were transfected with pMX construct of human Lin28A. Cells were analyzed ~62 hours after transfection.

• WB

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Western blot - Anti-Lin28A antibody [6D1F9] (AB76369)

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Western blot - Anti-Lin28A antibody [6D1F9] (ab76369) at 1/2000 dilution

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Cell lysates prepared from human NTERA-2 cells

Predicted band size: 23 kDa

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• sELISA

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Sandwich ELISA - Anti-Lin28A antibody [6D1F9] (AB76369)

Standard Curve for Lin28A (Analyte : (ab89225) Lin28 protein (His tag) (ab89225)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody (ab76369) Mouse monoclonal [6D1F9] to Lin28A (ab76369) at 0.2 ug/ml and Detector Antibody (ab63740) Rabbit polyclonal to Lin28A (ab63740) at 0.1 ug/ml.