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AB124765

Anti-Lin28A antibody [EPR4640]

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(14 Publications)

Rabbit Recombinant Monoclonal Lin28A antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

View Alternative Names

CSDD1, LIN28, ZCCHC1, LIN28A, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (AB124765)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (AB124765)

Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (AB124765)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (AB124765)

ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] (AB124765)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] (AB124765)

Intracellular Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (AB124765)
  • IP

Unknown

Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (AB124765)

ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab124765 & NCCIT whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (ab124765)

Predicted band size: 23 kDa

false

Western blot - Anti-Lin28A antibody [EPR4640] (AB124765)
  • WB

Unknown

Western blot - Anti-Lin28A antibody [EPR4640] (AB124765)

All lanes:

Western blot - Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution

Lane 1:

NCCIT cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

false

Western blot - Anti-Lin28A antibody [EPR4640] (AB124765)
  • WB

Lab

Western blot - Anti-Lin28A antibody [EPR4640] (AB124765)

All lanes:

Western blot - Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution

Lane 1:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Lin28A antibody [EPR4640]

  • 578 PE

    PE Anti-Lin28A antibody [EPR4640]

  • 660 APC

    APC Anti-Lin28A antibody [EPR4640]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Lin28A antibody [EPR4640]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Lin28A antibody [EPR4640]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Lin28A antibody [EPR4640]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Lin28A antibody [EPR4640]

  • Carrier free

    Anti-Lin28A antibody [EPR4640] - BSA and Azide free

  • HRP

    HRP Anti-Lin28A antibody [EPR4640]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4640

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/250", "IHCP-species-notes": "<p>(Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed : 21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). This results in the terminal uridylation of target pre-miRNAs (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed : 18951094, PubMed : 19703396, PubMed : 22118463, PubMed : 22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).
See full target information LIN28A
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadp0334 PubMed39292784

2024

Development of miRNA-based PROTACs targeting Lin28 for breast cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Xu,Xiaoran Zhao,Xingxing Liang,Dongyang Guo,Jing Wang,Qian Wang,Xinjing Tang

International journal of ophthalmology 16:1465-1474 PubMed37724283

2023

LIN28A attenuates high glucose-induced retinal pigmented epithelium injury through activating SIRT1-dependent autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Qing Yu,Song-Ping Yu,Jing Wu,Li-Na Lan,Bang-Xun Mao

Chinese medical journal 136:2484-2495 PubMed37433785

2023

Effect of down-regulation of let-7c/g on triggering a double-negative feedback loop and promoting restenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Xiaojun Zhou,Xianzhi Li,Shuai Yao,Shan Jiang,Rui Zhang,Zhiwei Zou,Lin Liao,Jianjun Dong

International journal of molecular sciences 24: PubMed37239986

2023

Antisense Oligonucleotides against Let-7 Enhance the Therapeutic Potential of Mesenchymal Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dae-Won Lee,Sungho Shin,Jeong-Ho Kim,Cheolju Lee,In Yong Kim,Il-Hoan Oh

Molecular psychiatry 26:7560-7580 PubMed34433918

2021

Cortical organoids model early brain development disrupted by 16p11.2 copy number variants in autism.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Urresti,Pan Zhang,Patricia Moran-Losada,Nam-Kyung Yu,Priscilla D Negraes,Cleber A Trujillo,Danny Antaki,Megha Amar,Kevin Chau,Akula Bala Pramod,Jolene Diedrich,Leon Tejwani,Sarah Romero,Jonathan Sebat,John R Yates Iii,Alysson R Muotri,Lilia M Iakoucheva

Cell reports 35:109198 PubMed34077720

2021

Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shlomi Dvir,Amir Argoetti,Chen Lesnik,Mark Roytblat,Kohava Shriki,Michal Amit,Tamar Hashimshony,Yael Mandel-Gutfreund

Frontiers in oncology 10:582669 PubMed33381451

2020

Long Non-Coding RNA LINC00355 Promotes the Development and Progression of Colorectal Cancer by Elevating Guanine Nucleotide Exchange Factor T Expression RNA Binding Protein lin-28 Homolog A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Bing Zhang,Ge Gao,Yinping Zhang,Qingxin Xia

International journal of biological sciences 16:1941-1953 PubMed32398961

2020

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yancheng Zhong,Lanqin Cao,Haotian Ma,Qian Wang,Pingpin Wei,Juan Yang,Yuqing Mo,Lihua Cao,Cijun Shuai,Shuping Peng

International journal of oncology 56:794-806 PubMed32124962

2020

H19/let‑7/Lin28 ceRNA network mediates autophagy inhibiting epithelial‑mesenchymal transition in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hanchu Xiong,Jianguo Shen,Zihan Chen,Jingjing Yang,Bojian Xie,Yunlu Jia,Ushani Jayasinghe,Ji Wang,Wenhe Zhao,Shuduo Xie,Linbo Wang,Jichun Zhou

Reproductive sciences (Thousand Oaks, Calif.) 27:1156-1163 PubMed32046465

2020

Overexpression of Lin28B Promoted the Proliferation of Adenomyotic Smooth Muscle Cells of the Junctional Zone via Regulating Let-7a.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Li Lin,Hua Duan,Sha Wang,Jin-Jiao Li
View all publications
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