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AB115698

Anti-Lin28B antibody

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(3 Publications)

Rabbit Polyclonal Lin28B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human LIN28B conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CSDD2, LIN28B, Protein lin-28 homolog B, Lin-28B

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)

Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Flow Cytometry (Intracellular) - Anti-Lin28B antibody (AB115698)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Lin28B antibody (AB115698)

Overlay histogram showing HL-60 cells stained with ab115698 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab115698, 1μg/1x106 cells) for 60 min at 37°C. The secondary antibody used was FITC conjugated goat anti-rabbitIgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)

Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB115698)

Immunohistochemistry Image of Human Spleen (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-Lin28B antibody (AB115698)
  • WB

Unknown

Western blot - Anti-Lin28B antibody (AB115698)

Incubation time was overnight at 4°C. Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lin28B antibody (ab115698) at 1/1000 dilution

All lanes:

HL60 cell line lysate at 35 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/5000 dilution

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human LIN28B conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6ZN17

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed : 22118463). Does not act on pri-miR21 (PubMed : 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed : 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed : 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
See full target information LIN28B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 13:141 PubMed34863268

2021

Circ-FBXW12 aggravates the development of diabetic nephropathy by binding to miR-31-5p to induce LIN28B.

Applications

Unspecified application

Species

Unspecified reactive species

Aidong Sun,Ningshuang Sun,Xiao Liang,Zhenbo Hou

Journal of cellular biochemistry 121:3720-3729 PubMed31713927

2019

LIN28B/IRS1 axis is targeted by miR-30a-5p and promotes tumor growth in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Tang,Jing Zhou,Lirui You,Zhirong Cui,Hui Zhang

Oncology letters 15:6975-6981 PubMed29725425

2018

Oncogene Lin28B increases chemosensitivity of colon cancer cells in a let-7-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Ma,Qi Zhao,Wenhao Chen,Yanqiao Zhang
View all publications
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Product promise

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