AB262859

Anti-Lin28B antibody

Name: Anti-Lin28B antibody (ab262859) | Abcam
Brand: Abcam Limited
SKU: AB262859
Price: 655 USD
Availability: InStock

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Rabbit Polyclonal Lin28B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LIN28B aa 200 to C-terminus.

View Alternative Names

CSDD2, LIN28B, Protein lin-28 homolog B, Lin-28B

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human testis tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. No expression shown as expected.

Immunocytochemistry/ Immunofluorescence - Anti-Lin28B antibody (AB262859)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lin28B antibody (AB262859)

PFA-fixed, Triton X-100 permeabilized human HEK-293 cells stained for Lin28B (green) using ab262859 at 4 μg/ml in ICC/IF. Localization to Nucleus, nucleoli and cytosol. Cytoskeleton counterstained in Red.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human liver tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. No expression shown as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human placenta tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human prostate tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. Low expression shown as expected.

Unknown

Western blot - Anti-Lin28B antibody (AB262859)

All lanes:

Western blot - Anti-Lin28B antibody (ab262859) at 0.4 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LIN28B aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6ZN17

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed : 22118463). Does not act on pri-miR21 (PubMed : 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed : 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed : 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

See full target information LIN28B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lin28B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Immunocytochemistry/ Immunofluorescence - Anti-Lin28B antibody (AB262859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Western blot - Anti-Lin28B antibody (AB262859)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

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