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AB184973

Anti-LIN37 antibody

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(1 Publication)

Rabbit Polyclonal LIN37 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LIN37 aa 150 to C-terminus.

View Alternative Names

MSTP064, LIN37, Protein lin-37 homolog, Antolefinin

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-LIN37 antibody (AB184973)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LIN37 antibody (AB184973)

Immunofluorescence analysis of U-251 MG (human brain glioma cell line) whole cellls labeling LIN37 in the nucleoplasm and mitochondria with ab184973 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LIN37 with ab184973 at a 1/20 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)

Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling LIN37 with ab184973 at a 1/20 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (AB184973)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling LIN37 with ab184973 at a 1/20 dilution.

Western blot - Anti-LIN37 antibody (AB184973)
  • WB

Unknown

Western blot - Anti-LIN37 antibody (AB184973)

All lanes:

Western blot - Anti-LIN37 antibody (ab184973) at 0.4 µg/mL

All lanes:

Human cell line HDLM-2

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LIN37 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96GY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIN37 also known as Lin-37 DREAM complex component or 37 kDa protein is a component of the DREAM (DP RB-like E2F and MuvB) complex. This protein weighs approximately 37 kDa. It is predominantly expressed in proliferating tissues and plays an important role in controlling cell cycle progression. LIN37 localizes mainly to the nucleus where it carries out its function.
Biological function summary

LIN37 operates as part of the DREAM complex which is essential for regulating cell cycle-dependent gene expression. The DREAM complex consists of other proteins such as LIN9 LIN52 and RBL1. LIN37 helps repress genes that promote DNA replication and mitosis during the quiescent phase where cells are not actively dividing. By cooperating with other complex members it helps maintain cellular homeostasis and prevent unnecessary cell division.

Pathways

LIN37 and its role in the DREAM complex impact the control of the Rb-E2F pathway. This pathway regulates the transcription of genes required for cell cycle progression. Through the interplay with RBL1 and E2F4 LIN37 contributes to cell cycle exit and entry ensuring the cells only progress to DNA synthesis when necessary. The complex finely tunes cell cycle events to maintain balanced proliferation.

LIN37 has a noted association with certain cancers such as retinoblastoma. Disruption in the DREAM complex including LIN37 function may lead to derepression of genes necessary for DNA synthesis and mitosis contributing to uncontrolled cell proliferation. LIN37's interactions with proteins like RBL2 emphasize its significance in tumor suppression presenting potential therapeutic targets for cancer interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LIN37
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 27:25 PubMed38125354

2023

Identification of potential miR‑155 target genes in epidermal immune microenvironment of atopic dermatitis patients and their inflammatory effects on HaCaT cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Wang,Lu Chen,Xiaoqing Chen,Chang Liu,Wenhong Qiu,Kaiwen Guo
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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