AB138425

Anti-LIN54 antibody

Name: Anti-LIN54 antibody (ab138425) | Abcam
Brand: Abcam Limited
SKU: AB138425

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(2 Publications)

Rabbit Polyclonal LIN54 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LIN54 aa 50-100.

View Alternative Names

CXCDC1, KIAA2037, LIN54, Protein lin-54 homolog, CXC domain-containing protein 1

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Immunoprecipitation - Anti-LIN54 antibody (AB138425)

Detection of LIN54 by immunoprecipitation in HeLa whole cell lysate using ab138425 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). A different antibody against Human LIN54 was used for Western Blot detection step. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-LIN54 antibody (ab138425)

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human LIN54 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q6MZP7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99.01% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIN54 also known as protein LINT-54 is a component of the DREAM complex involved in cell cycle regulation. This protein has a molecular weight of around 70 kDa and is expressed in various tissues throughout the body including the brain and lungs. LIN54 contains specific DNA-binding domains that enable it to interact directly with DNA allowing it to regulate the transcription of target genes. By binding to specific DNA sequences this protein helps control key biological processes necessary for cell proliferation.

Biological function summary

In coordination with other proteins LIN54 plays an important role in the regulation of the cell cycle. It forms part of the multiprotein DREAM complex a regulatory unit that shifts cells from proliferation to a quiescent state by repressing cell cycle-dependent genes. During the cell cycle LIN54 supports transitions between different phases by regulating gene expression connected to cell cycle arrest and activation ensuring cells divide correctly and avoid errors that could lead to pathological states.

Pathways

Multiple cell cycle pathways involve the participation of LIN54 influencing cellular transitions and growth signals. LIN54 is significantly associated with the E2F transcription factor pathway which regulates genes required for cell cycle progression. Another pathway that LIN54 plays a part in is the p53 signaling pathway where it interacts with proteins like RB1. These pathways help maintain cellular homeostasis and prevent unregulated growth that may result in various diseases.

LIN54 shows connections to cancer and neurodegenerative diseases. Aberrant expression or regulation of LIN54 could lead to uncontrollable cell proliferation seen in cancers. One of its associations in cancer involves the interaction with RB1 commonly mutated in various cancers. Additionally dysregulation in the DREAM complex including LIN54 might relate to neurodegenerative diseases like Alzheimer's where cell cycle control in neurons is disrupted. Understanding LIN54's role in these diseases could help develop therapeutic strategies targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context (PubMed : 17531812, PubMed : 17671431). In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes (PubMed : 17531812, PubMed : 17671431). In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation (PubMed : 17531812, PubMed : 17671431). In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner (PubMed : 19725879). Specifically recognizes the consensus motif 5'-TTYRAA-3' in target DNA (PubMed : 27465258).

See full target information LIN54

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 15:3350-3358 PubMed37426130

2023

Overexpressed collaborates with MMB to increase inhibitor sensitivity in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tan,Jingbo Ma,Weiqiang Zhang,Jing Zhao,Keqiang Liu

Virus research 189:206-13 PubMed24911240

2014

Success of measles virotherapy in ATL depends on type I interferon secretion and responsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

M Cecilia M Parrula,Soledad A Fernandez,Kristina Landes,Devra Huey,Michael Lairmore,Stefan Niewiesk

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LIN54 antibody

Immunoprecipitation - Anti-LIN54 antibody (AB138425)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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