AB127049

Anti-LIN7 antibody

Name: Anti-LIN7 antibody (ab127049) | Abcam
Brand: Abcam Limited
SKU: AB127049

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(1 Publication)

Rabbit Polyclonal LIN7A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LIN7A aa 1-200.

View Alternative Names

MALS1, VELI1, LIN7A, Protein lin-7 homolog A, Lin-7A, hLin-7, Mammalian lin-seven protein 1, Tax interaction protein 33, Vertebrate lin-7 homolog 1, MALS-1, TIP-33, Veli-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-LIN7 antibody (AB127049)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LIN7 antibody (AB127049)

Immunofluorescence analysis of MCF-7 cells fixed in ice-cold MeOH for 5 min labelling LIN7A protein at cell membrane using ab127049 at a 1/1000 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

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Western blot - Anti-LIN7 antibody (AB127049)

12% SDS PAGE

All lanes:

Western blot - Anti-LIN7 antibody (ab127049) at 1/5000 dilution

All lanes:

MCF7 whole cell lysate at 30 µg

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LIN7A aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O14910

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIN7 also known as LIN7A LIN7B and LIN7C is a protein with a molecular mass of about 24-28 kDa. LIN7 proteins are present in the brain kidney and epithelial cells where they work to regulate cellular processes. The three versions of LIN7 A B and C are highly similar in structure and function within these tissues helping maintain cell polarity and joining protein networks together at synapses and cell junctions.

Biological function summary

LIN7 proteins form part of a multi-protein complex that stabilizes surface molecules in polarized cells. In neurons LIN7 interacts with other proteins to ensure proper synaptic transmission. It interacts with the PDZ domain-containing proteins that organize proteins at specific cell locations. This organization is fundamental for neurotransmitter receptor localization especially sticking closely to membrane proteins like NMDA receptors and potassium channels which are very important for neuronal signaling.

Pathways

LIN7 proteins play an important role in both the asymmetric cell division pathway and the cell signaling pathway. In the asymmetric cell division pathway LIN7 connects with other proteins such as CRB3 and CASK assisting in the proper orientation and division of cells. The cell signaling pathway involves LIN7's association with other proteins like DLG and MPP7 helping transmit signals that control cellular growth and behavior emphasizing the role LIN7 performs in different cellular environments.

LIN7 proteins show a connection with neurological disorders and certain types of cancer. For instance mutations or dysregulation of LIN7 can be linked to cognitive disorders due to their role in synaptic function and neural communication as witnessed in autism spectrum disorders. Additionally altered expression of LIN7 might associate with some epithelial cancers where disrupted cell polarity is a feature. LIN7 connects to the E-cadherin protein in cancer progression highlighting its importance in maintaining tissue organization and cell-to-cell adhesion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity). This complex may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

See full target information LIN7A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 132: PubMed30890647

2019

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Tiina Viita,Salla Kyheröinen,Bina Prajapati,Jori Virtanen,Mikko J Frilander,Markku Varjosalo,Maria K Vartiainen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIN7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-LIN7 antibody (AB127049)

Western blot - Anti-LIN7 antibody (AB127049)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Cell Lines & Lysates

Secondary Antibodies

Primary Antibodies

Product promise