AB174297

Anti-LIN7A antibody [EPR12609]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal LIN7A antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

View Alternative Names

MALS1, VELI1, LIN7A, Protein lin-7 homolog A, Lin-7A, hLin-7, Mammalian lin-seven protein 1, Tax interaction protein 33, Vertebrate lin-7 homolog 1, MALS-1, TIP-33, Veli-1

2 Images

Supplier Data

Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (AB174297)

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab174297 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (ab174297)

Predicted band size: 26 kDa

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Supplier Data

Western blot - Anti-LIN7A antibody [EPR12609] (AB174297)

All lanes:

Western blot - Anti-LIN7A antibody [EPR12609] (ab174297) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

false

Carrier free

Anti-LIN7A antibody [EPR12609] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12609

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIN7A also known as Lin-7 homolog A or MALS-1 is a protein with a molecular weight of approximately 25 kDa. It plays a significant mechanical role in cell signaling and synaptic assembly. LIN7A primarily localizes at cell membranes with pronounced expression in the brain and heart where it participates in the development and maintenance of cellular polarity and tight junctions.

Biological function summary

LIN7A is involved in neuronal signaling and synaptic function. It forms part of the larger complex known as the membrane-associated guanylate kinase (MAGUK) protein family. In neurons LIN7A maintains receptor localization by interacting with synaptic proteins and is essential in stabilizing various complexes necessary for synaptic communication.

Pathways

LIN7A participates significantly in the MAPK and PI3K/Akt signaling pathways. These pathways are important in regulating cell growth and survival. LIN7A interacts with other proteins like DLG1 and CASK which are essential components in maintaining cellular signaling integrity within these pathways.

Disruptions in LIN7A expression or function have connections with neurological disorders such as schizophrenia and autism spectrum disorders. LIN7A’s interaction with other proteins such as DLG4 suggests its involvement in synaptic dysfunctions which are common characteristics in these conditions. Understanding LIN7A's roles in these disorders can potentially lead to targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity). This complex may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

See full target information LIN7A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 15:37 PubMed36864492

2023

DNA methylation landscape reveals LIN7A as a decitabine-responsive marker in patients with t(8;21) acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shujiao He,Yan Li,Xuanren Shi,Lei Wang,Diya Cai,Jingfeng Zhou,Li Yu

Cell death & disease 9:535 PubMed29749382

2018

microRNA-501-3p suppresses metastasis and progression of hepatocellular carcinoma through targeting LIN7A.

Applications

Unspecified application

Species

Unspecified reactive species

Chubin Luo,Dan Yin,Hao Zhan,Uyunbilig Borjigin,Chuanjiang Li,Zhengjun Zhou,Zhiqiang Hu,Pengcheng Wang,Qiman Sun,Jia Fan,Jian Zhou,Xin Wang,Shaolai Zhou,Xiaowu Huang

American journal of physiology. Lung cellular and 313:L889-L898 PubMed28798257

2017

Aquaporin 1-mediated changes in pulmonary arterial smooth muscle cell migration and proliferation involve β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yun,Haiyang Jiang,Ning Lai,Jian Wang,Larissa A Shimoda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIN7A antibody [EPR12609]

Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (AB174297)

Western blot - Anti-LIN7A antibody [EPR12609] (AB174297)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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