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Rabbit Recombinant Monoclonal LIN7A antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

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Images

Western blot - Anti-LIN7A antibody [EPR12609] (AB174297), expandable thumbnail
  • Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (AB174297), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended
Rat
Not recommended
Expected
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity). This complex may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

Alternative names

MALS1, VELI1, LIN7A, Protein lin-7 homolog A, Lin-7A, hLin-7, Mammalian lin-seven protein 1, Tax interaction protein 33, Vertebrate lin-7 homolog 1, MALS-1, TIP-33, Veli-1

Recommended products

Rabbit Recombinant Monoclonal LIN7A antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12609
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LIN7A also known as Lin-7 homolog A or MALS-1 is a protein with a molecular weight of approximately 25 kDa. It plays a significant mechanical role in cell signaling and synaptic assembly. LIN7A primarily localizes at cell membranes with pronounced expression in the brain and heart where it participates in the development and maintenance of cellular polarity and tight junctions.

Biological function summary

LIN7A is involved in neuronal signaling and synaptic function. It forms part of the larger complex known as the membrane-associated guanylate kinase (MAGUK) protein family. In neurons LIN7A maintains receptor localization by interacting with synaptic proteins and is essential in stabilizing various complexes necessary for synaptic communication.

Pathways

LIN7A participates significantly in the MAPK and PI3K/Akt signaling pathways. These pathways are important in regulating cell growth and survival. LIN7A interacts with other proteins like DLG1 and CASK which are essential components in maintaining cellular signaling integrity within these pathways.

Associated diseases and disorders

Disruptions in LIN7A expression or function have connections with neurological disorders such as schizophrenia and autism spectrum disorders. LIN7A’s interaction with other proteins such as DLG4 suggests its involvement in synaptic dysfunctions which are common characteristics in these conditions. Understanding LIN7A's roles in these disorders can potentially lead to targeted therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-LIN7A antibody [EPR12609] (ab174297), expandable thumbnail

    Western blot - Anti-LIN7A antibody [EPR12609] (ab174297)

    All lanes: Western blot - Anti-LIN7A antibody [EPR12609] (ab174297) at 1/1000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: Human fetal brain lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (ab174297), expandable thumbnail

    Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (ab174297)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab174297 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-LIN7A antibody [EPR12609] (ab174297)

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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