Name: Anti-LINE-1 ORF1p antibody [EPR21844-108]
SKU: ab216324
Rating: 5 (2 reviews)

Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) is a rabbit monoclonal antibody detecting LINE-1 ORF1p in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency

- Over 10 publications

Images

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR21844-108] (AB216324), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P	IHC-Fr
Mouse	Tested	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1980	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Associated Products

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Target data

See full target information Lire1

Function

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

Alternative names

LINE-1 retrotransposable element ORF1 protein, L1-ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1, Transposase element L1Md-A101/L1Md-A102/L1Md-A2, Lire1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21844-108
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP, WB in mouse samples.
Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) specifically detects LINE-1 ORF1p (UniProt ID: P11260; Molecular weight: 41kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) has been cited over 12 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR21844-108 - ab245122.
Antibody clone EPR21844-108 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 (ab303593).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The LINE-1 ORF1p also called L1 ORF1p is a protein encoded by the open reading frame 1 (ORF1) of the long interspersed nuclear element-1 (LINE-1 or L1) family. These proteins typically have a mass of around 40 kDa. They are predominantly expressed in the germ line and embryonic tissues but can also be found in various somatic tissues under abnormal conditions. L1 ORF1p plays a critical role in the retrotransposition process by forming ribonucleoprotein complexes that assist LINE-1 RNA in entering the nucleus and integrating into the genome.

Biological function summary

L1 ORF1p facilitates the mobilization of LINE-1 elements within the genome impacting genome stability and variability. This protein forms a trimeric complex that binds LINE-1 RNA protecting it from degradation. By promoting the retrotransposition of LINE-1 elements L1 ORF1p influences genomic dynamics potentially contributing to genetic diversity but also potentially causing insertions that may lead to genomic instability and associated pathologies.

Pathways

The activity of L1 ORF1p intersects with genome defense mechanisms and cellular stress response pathways. Important pathways include the single-stranded DNA break repair system and retrotransposon silencing mechanisms where proteins like TREX1 and MOV10 play related roles. These pathways help control the activity of transposable elements and maintain genomic integrity providing a first line of defense against potential disruptions caused by retrotransposition events.

Associated diseases and disorders

Aberrant expression or dysregulation of L1 ORF1p has been linked to cancer and neurodegenerative disorders. Elevated levels of L1 ORF1p have been observed in some cancers such as lung and breast cancer where it may contribute to genomic instability. Furthermore Alzheimer's disease has shown L1 activity potentially involving interaction with amyloid precursor protein (APP) and contributing to disease pathology. Understanding these associations highlights the relevance of L1 ORF1p as a potential target for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling LINE-1 ORF1p with ab216324 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in F9 cell line.
Negative control: NIH/3T3 (PMID: 24324740).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Blocking/Dilution buffer: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID:24324740).
All lanes: Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) at 1/1000 dilution
Lane 1: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3min
Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324) at 1/1000 dilution
All lanes: mouse testis lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining on mouse liver (PMID: 24607009) is observed. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
LINE-1 ORF1p was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate with ab216324 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216324 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5,000 dilution
Lane 1: F9 whole cell lysate 10 μg (Input).
Lane 2: ab216324 IP in F9 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216324 in F9 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Predicted band size: 44 kDa
Observed band size: 41 kDa, 43 kDa
Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / F9 (mouse embryonal carcinoma epithelial cell, Right) cell lines labeling LINE-1 ORF1p with ab216324 at 1/500 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: NIH/3T3 (Reference for the negative control is PMID: 24324740).
Immunohistochemistry (Frozen sections) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
ab216324 staining LINE-1 ORF1p in Wt and Maelstrom (Mael) null mutant mouse testis by Immunohistochemistry (Frozen sections). Mael is a repressor of LINE-1 transposable elements, so the expression of LINE-1 ORF1p increases in this KO. Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; Samples were incubated with primary antibody (1/100, 1/500 and 1/1000) for 12 hours at 4°C in 3% Donkey Serum in PBS + 0.05% Triton X. Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150073) (1/1000) was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR21844-108] (ab216324)
Flow cytometry overlay histogram showing left F9 positive cells and right negative NIH3T3 stained with ab216324 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 mins. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab216324) (1x 10⁶ in 100μl at 1.0μg/ml (1/1980 dilution)) for 30 mins at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins at 22°C
Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in F9 Fixed with 80% methanol (5 mins) / permeabilised with 0.1% PBS-Triton X-100 for 15 minss under the same conditions.