AB230966

Anti-LINE-1 ORF1p antibody [EPR22227-54]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal LINE-1 ORF1p antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

LRE1, L1RE1, LINE-1 retrotransposable element ORF1 protein, L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue (Panel A) and adjacent human normal colon tissue (Panel B) labeling LINE-1 ORF1p with ab230966 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in colon cancer (Panel A) but negative in adjacent normal tissue (Panel B). (PMID : 24607009) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonic carcinoma cell line) cells labeling LINE-1 ORF1p with ab230966 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic with punctate foci and nucleolar staining in NCCIT cells (PMID : 15028673; 17562864). The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue (Panel A) and adjacent human normal pancreatic tissue (Panel B) labeling LINE-1 ORF1p with ab230966 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in pancreatic cancer (Panel A) but negative in adjacent normal tissue (Panel B). (PMID : 24607009) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling LINE-1 ORF1p with ab230966 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic with punctate foci and nucleolar staining in MCF7 cells (PMID : 15028673; 17562864). The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HCT 116 (human colorectal carcinoma cell line) (Left panel)and NCCIT (human pluripotent embryonic carcinoma cell line) (Right panel) cells labeling LINE-1 ORF1p with ab230966 at 1/500 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^ï¿½488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control : The expression level on HCT 116 (low) and NCCIT (high) is consistent with literature (PMID : 27016617).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (Panel A) and adjacent human normal lung tissue (Panel B) labeling LINE-1 ORF1p with ab230966 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in lung cancer (Panel A) but negative in adjacent normal tissue (Panel B) (PMID : 24607009) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

LINE-1 ORF1p was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab230966 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230966 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : NCCIT whole cell lysate 10 μg (Input).
Lane 2 : ab230966 IP in NCCIT whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230966 in NCCIT whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

The lower 33 kDa band is likely to be a truncated form (PMID : 29505568).

All lanes:

Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-54] (ab230966)

Predicted band size: 43 kDa

Observed band size: 33 kDa,42 kDa

false

Unknown

Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1 and 2 : 37 seconds.

Lanes 3 and 4 : 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29505568).

The lower 33 kDa band is likely to be a truncated form (PMID : 29505568).

Control cell line : HCT 116 (low-expression, PMID : 27016617).

All lanes:

Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-54] (ab230966) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 33 kDa,42 kDa

false

Carrier free

Anti-LINE-1 ORF1p antibody [EPR22227-54] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22227-54

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LINE-1 ORF1p also called L1 ORF1p is a protein encoded by the open reading frame 1 (ORF1) of the long interspersed nuclear element-1 (LINE-1 or L1) family. These proteins typically have a mass of around 40 kDa. They are predominantly expressed in the germ line and embryonic tissues but can also be found in various somatic tissues under abnormal conditions. L1 ORF1p plays a critical role in the retrotransposition process by forming ribonucleoprotein complexes that assist LINE-1 RNA in entering the nucleus and integrating into the genome.

Biological function summary

L1 ORF1p facilitates the mobilization of LINE-1 elements within the genome impacting genome stability and variability. This protein forms a trimeric complex that binds LINE-1 RNA protecting it from degradation. By promoting the retrotransposition of LINE-1 elements L1 ORF1p influences genomic dynamics potentially contributing to genetic diversity but also potentially causing insertions that may lead to genomic instability and associated pathologies.

Pathways

The activity of L1 ORF1p intersects with genome defense mechanisms and cellular stress response pathways. Important pathways include the single-stranded DNA break repair system and retrotransposon silencing mechanisms where proteins like TREX1 and MOV10 play related roles. These pathways help control the activity of transposable elements and maintain genomic integrity providing a first line of defense against potential disruptions caused by retrotransposition events.

Aberrant expression or dysregulation of L1 ORF1p has been linked to cancer and neurodegenerative disorders. Elevated levels of L1 ORF1p have been observed in some cancers such as lung and breast cancer where it may contribute to genomic instability. Furthermore Alzheimer's disease has shown L1 activity potentially involving interaction with amyloid precursor protein (APP) and contributing to disease pathology. Understanding these associations highlights the relevance of L1 ORF1p as a potential target for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

See full target information L1RE1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6327 PubMed40645931

2025

Opposing roles of pseudokinases NRBP1 and NRBP2 in regulating L1 retrotransposition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Shaobo Cong,Ruoyao Li,Jennifer Schwarz,Thilo Schulze,Raban A Gevelhoff,Xinyan Chen,Sara Ullrich,Kristina Falkenstein,Denis Ott,Pia Eixmann,Angelica Trentino,Antje Thien,Thierry Heidmann,Ekkehard Schulze,Bettina Warscheid,Ralf Baumeister,Wenjing Qi

Immunity 57:2879-2894.e11 PubMed39577413

2024

Cancer cells restrict immunogenicity of retrotransposon expression via distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Sun,Eunae You,Jungeui Hong,David Hoyos,Isabella Del Priore,Kaloyan M Tsanov,Om Mattagajasingh,Andrea Di Gioacchino,Sajid A Marhon,Jonathan Chacon-Barahona,Hao Li,Hua Jiang,Samira Hozeifi,Omar Rosas-Bringas,Katherine H Xu,Yuhui Song,Evan R Lang,Alexandra S Rojas,Linda T Nieman,Bidish K Patel,Rajmohan Murali,Pharto Chanda,Ali Karacay,Nicolas Vabret,Daniel D De Carvalho,Daniel Zenklusen,John LaCava,Scott W Lowe,David T Ting,Christine A Iacobuzio-Donahue,Alexander Solovyov,Benjamin D Greenbaum

Nature 626:1116-1124 PubMed38355802

2024

Autonomous transposons tune their sequences to ensure somatic suppression.

Applications

Unspecified application

Species

Unspecified reactive species

İbrahim Avşar Ilık,Petar Glažar,Kevin Tse,Björn Brändl,David Meierhofer,Franz-Josef Müller,Zachary D Smith,Tuğçe Aktaş

Nucleic acids research 51:4867-4880 PubMed36942479

2023

LINE-1 repression in Epstein-Barr virus-associated gastric cancer through viral-host genome interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyu Zhang,Weikang Sun,Xiaoxin You,Dongge Xu,Lingling Wang,Jingping Yang,Erguang Li,Susu He

Cancer immunology, immunotherapy : CII 72:985-1001 PubMed36251028

2022

Peptide vaccine from cancer-testis antigen ODF2 can potentiate the cytotoxic T lymphocyte infiltration through IL-15 in non-MSI-H colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Shi,Xiuman Zhou,Liwei Pang,Mingshuang Wang,Yubing Li,Chunxia Chen,Haoming Ning,Lihan Zhang,Guangxing Yue,Lu Qiu,Wenshan Zhao,Yuanming Qi,Yahong Wu,Yanfeng Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LINE-1 ORF1p antibody [EPR22227-54]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-54] (AB230966)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise