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Rabbit Recombinant Monoclonal LINE-1 ORF1p antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249), expandable thumbnail
  • Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

Alternative names

LINE-1 retrotransposable element ORF1 protein, L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1, LRE1, L1RE1

Recommended products

Rabbit Recombinant Monoclonal LINE-1 ORF1p antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22227-6

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The LINE-1 ORF1p also called L1 ORF1p is a protein encoded by the open reading frame 1 (ORF1) of the long interspersed nuclear element-1 (LINE-1 or L1) family. These proteins typically have a mass of around 40 kDa. They are predominantly expressed in the germ line and embryonic tissues but can also be found in various somatic tissues under abnormal conditions. L1 ORF1p plays a critical role in the retrotransposition process by forming ribonucleoprotein complexes that assist LINE-1 RNA in entering the nucleus and integrating into the genome.

Biological function summary

L1 ORF1p facilitates the mobilization of LINE-1 elements within the genome impacting genome stability and variability. This protein forms a trimeric complex that binds LINE-1 RNA protecting it from degradation. By promoting the retrotransposition of LINE-1 elements L1 ORF1p influences genomic dynamics potentially contributing to genetic diversity but also potentially causing insertions that may lead to genomic instability and associated pathologies.

Pathways

The activity of L1 ORF1p intersects with genome defense mechanisms and cellular stress response pathways. Important pathways include the single-stranded DNA break repair system and retrotransposon silencing mechanisms where proteins like TREX1 and MOV10 play related roles. These pathways help control the activity of transposable elements and maintain genomic integrity providing a first line of defense against potential disruptions caused by retrotransposition events.

Associated diseases and disorders

Aberrant expression or dysregulation of L1 ORF1p has been linked to cancer and neurodegenerative disorders. Elevated levels of L1 ORF1p have been observed in some cancers such as lung and breast cancer where it may contribute to genomic instability. Furthermore Alzheimer's disease has shown L1 activity potentially involving interaction with amyloid precursor protein (APP) and contributing to disease pathology. Understanding these associations highlights the relevance of L1 ORF1p as a potential target for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling LINE-1 ORF1p with ab245249 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic with punctate foci and nucleolar staining in NCCIT cells (PMID: 17562864; 15028673). The nuclear counter stain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling LINE-1 ORF1p with ab245249 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic with punctate foci and nucleolar staining in MCF7 cells (PMID: 15028673; 17562864). The nuclear counter stain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution..

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Immunohistochemical analysis of paraffin-embedded human colon cancer (Panel A) and adjacent normal tissue (Panel B) labeling LINE-1 ORF1p with ab245249 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in colon cancer (Panel A) but negative in adjacent normal tissue (Panel B). (PMID:24607009). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    LINE-1 ORF1p was immunoprecipitated from 0.35 mg NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate with ab245249 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245249 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: NCCIT whole cell lysate 10 μg (Input).
    Lane 2: ab245249 IP in NCCIT whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab245249 in NCCIT whole cell lysate.

    The lower 33-kDa band is likely to be the truncated form (PMID: 29505568).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Observed band size: 33 kDa, 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer (Panel A) and adjacent normal tissue (Panel B) labeling LINE-1 ORF1p with ab245249 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in pancreatic cancer (Panel A) but negative in adjacent normal tissue (Panel B). (PMID:24607009). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Immunohistochemical analysis of paraffin-embedded human lung cancer (Panel A) and adjacent normal tissue (Panel B) labeling LINE-1 ORF1p with ab245249 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in lung cancer (Panel A) but negative in adjacent normal tissue (Panel B). (PMID:24607009). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 29505568).

    The lower 33-kDa band is likely to be a truncated form (PMID: 29505568).

    Control cell line: HCT-116 (low-expression, PMID: 27016617).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds; Lane 2, 3 and 4: 37 seconds.

    All lanes: Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249) at 1/1000 dilution

    Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 4: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa

    Observed band size: 33 kDa, 42 kDa

  • Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LINE-1 ORF1p antibody [EPR22227-6] (ab245249)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left panel) / NCCIT (human pluripotent embryonic carcinoma epithelial cell, Right panel) cell lines labeling LINE-1 ORF1p with ab245249 at 1/500 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    The expression level on HCT 116 (low) and NCCIT (high) is consistent with literature (PMID: 27016617).

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Product protocols

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