Rabbit Recombinant Monoclonal LINE-1 ORF2p antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (AB263435), expandable thumbnail](https://content.abcam.com/products/images/line-1-orf2p-antibody-mt49-bsa-and-azide-free-ab263435--immunocytochemistry-immunofluorescence-img422630.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (AB263435)")
![Western blot - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (AB263435), expandable thumbnail](https://content.abcam.com/products/images/line-1-orf2p-antibody-mt49-bsa-and-azide-free-ab263435--western-blot-img422629.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (AB263435)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Has a reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Has also an endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome.
Alternative names
LINE-1 retrotransposable element ORF2 protein, ORF2p
Recommended products
Rabbit Recombinant Monoclonal LINE-1 ORF2p antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.
Alternative names
LINE-1 retrotransposable element ORF2 protein, ORF2p
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Carrier free
Yes
- Clone number
MT49
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab263435 is the carrirer-free version of Anti-LINE-1 ORF2p antibody [MT49] ab263071.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435), expandable thumbnail](https://content.abcam.com/products/images/line-1-orf2p-antibody-mt49-bsa-and-azide-free-ab263435--immunocytochemistry-immunofluorescence-img422630.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435)")
Immunocytochemistry/ Immunofluorescence - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435)
This data was developed using Anti-LINE-1 ORF2p antibody [MT49] ab263071 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with a LINE-1 ORF2p expression vector containing a myc tag. cells labelling LINE-1 ORF2p with Anti-LINE-1 ORF2p antibody [MT49] ab263071 at 1/2000 (0.506 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in 293T cells transfected with LINE-1 ORF2p protein expression vector containing a myc tag, no staining on HEK-293T transfected with empty vector containing a myc-tag.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 647 Anti-Myc tag antibody [9E10] ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Western blot - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435), expandable thumbnail](https://content.abcam.com/products/images/line-1-orf2p-antibody-mt49-bsa-and-azide-free-ab263435--western-blot-img422629.jpg "Western blot - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435)")
Western blot - Anti-LINE-1 ORF2p antibody [MT49] - BSA and Azide free (ab263435)
This data was developed using Anti-LINE-1 ORF2p antibody [MT49] ab263071, the same antibody clone in a different buffer formulation.
The identity of the lower MW band at approximately 40kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LINE-1 ORF2p antibody [MT49] (Anti-LINE-1 ORF2p antibody [MT49] ab263071) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human LINE-1 ORF2p expression vector containing a His-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa, 36 kDa
Exposure time: 15s
The identity of the lower MW band at approximately 40kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
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