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AB249518

Anti-LINGO3 antibody [EPR9635] - BSA and Azide free

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Rabbit Recombinant Monoclonal LINGO3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

LERN2, LRRN6B, LINGO3, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 3, Leucine-rich repeat neuronal protein 2, Leucine-rich repeat neuronal protein 6B

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)

This data was developed using ab169772, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling LINGO3 with ab169772 at 1/250 dilution (green). DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)

This data was developed using ab169772, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized SH-SY5Y cells labeling LINGO3 with ab169772 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)
  • WB

Unknown

Western blot - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)

This data was developed using ab169772, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LINGO3 antibody [EPR9635] (<a href='/en-us/products/primary-antibodies/lingo3-antibody-epr9635-ab169772'>ab169772</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

NCI-H460 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

false

Immunoprecipitation - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)
  • IP

Unknown

Immunoprecipitation - Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (AB249518)

This data was developed using ab169772, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab169772.

All lanes:

Immunoprecipitation - Anti-LINGO3 antibody [EPR9635] (<a href='/en-us/products/primary-antibodies/lingo3-antibody-epr9635-ab169772'>ab169772</a>)

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9635

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249518 is the carrier-free version of ab169772.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LINGO3 also known as Leucine-Rich Repeat and Ig Domain-Containing contributes to regulating neuronal survival and function. LINGO3 has a molecular mass of approximately 87 kDa and shows expression mostly in the central nervous system. It belongs to the LINGO family which includes several proteins that share overlapping functions in the nervous system.
Biological function summary

LINGO3 plays a significant role in the development and maintenance of neuronal circuits. It often partakes in protein complexes that influence nerve growth and guidance. Its action affects cellular functions such as differentiation and axonal growth contributing to the proper configuration of neural networks.

Pathways

LINGO3 engages with mechanisms involved in neuronal signal transduction and myelination. It operates within the axon guidance pathway and the Nogo receptor pathway. In these pathways proteins such as RTN4 (also known as Nogo) interact with LINGO3 to modulate neuronal growth and inhibition of neurite outgrowth.

LINGO3's activity could connect with multiple sclerosis and neurodegenerative conditions. The dysregulation of LINGO3 affects axonal integrity and remyelination processes which are important in the progression of these disorders. LINGO3 relates to proteins like Nogo and MAG which are pivotal in neuropathological conditions impacting myelin stability and repair.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LINGO3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com