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AB249823

Anti-LINGO4 antibody [EPR12600] - BSA and Azide free

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Rabbit Recombinant Monoclonal LINGO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

LRRN6D, UNQ9248/PRO34002, LINGO4, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 4, Leucine-rich repeat neuronal protein 6D

2 Images
Western blot - Anti-LINGO4 antibody [EPR12600] - BSA and Azide free (AB249823)
  • WB

Supplier Data

Western blot - Anti-LINGO4 antibody [EPR12600] - BSA and Azide free (AB249823)

This data was developed using ab174832, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LINGO4 antibody [EPR12600] (<a href='/en-us/products/primary-antibodies/lingo4-antibody-epr12600-ab174832'>ab174832</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 64 kDa

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Immunoprecipitation - Anti-LINGO4 antibody [EPR12600] - BSA and Azide free (AB249823)
  • IP

Supplier Data

Immunoprecipitation - Anti-LINGO4 antibody [EPR12600] - BSA and Azide free (AB249823)

This data was developed using ab174832, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate labeling LINGO4, immunoprecipitated using ab174832 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-LINGO4 antibody [EPR12600] (<a href='/en-us/products/primary-antibodies/lingo4-antibody-epr12600-ab174832'>ab174832</a>)

Predicted band size: 59 kDa,64 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12600

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249823 is the carrier-free version of ab174832.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LINGO4 also known as Leucine Rich Repeat and Ig domain containing 4 is a protein with a mass of approximately 78 kDa. This protein is part of the LRR transmembrane protein family and plays an important role as a modulator in the nervous system. Expression of LINGO4 is detected in the central nervous system particularly in neural tissues. The protein's structure includes leucine-rich repeats that facilitate protein-protein interactions.
Biological function summary

LINGO4 functions as an important regulator in neural development and axon formation. It interacts with other proteins such as the Nogo receptor forming multiprotein complexes that influence neuronal growth. Through these complexes LINGO4 negatively regulates nerve regeneration and neuronal survival impacting the growth and maintenance of the nervous system. This regulation is essential during neural maturation and response to injury.

Pathways

LINGO4 plays a significant role in neuronal signaling pathways such as the Nogo receptor signaling pathway and the myelin inhibitory signaling pathway. In these pathways LINGO4 interacts with other proteins like NOGO and p75 neurotrophin receptor modulating neuronal growth inhibition. These interactions and pathways are vital for controlling neural plasticity and regeneration impacting the nervous system's ability to recover from damage.

LINGO4's function is connected to neurological conditions including multiple sclerosis and spinal cord injuries. Altered expression or dysfunction of LINGO4 can impair neural repair processes contributing to disease pathology. In disorders like multiple sclerosis the protein's interaction with the Nogo receptor and the myelin-associated glycoprotein pathways plays a pivotal role in modulating the immune responses and neuronal damage influencing disease progression and patient outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LINGO4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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