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AB217360

Anti-LIPC antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal LIPC antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LIPC aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HTGL, LIPC, Hepatic triacylglycerol lipase, HL, Hepatic lipase, Lipase member C, Lysophospholipase, Phospholipase A1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPC antibody (AB217360)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPC antibody (AB217360)

Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded Human liver carcinoma tissue labeling LIPC with ab217360 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.

Antigen retrieval by boiling in sodium citrate buffer (pH 6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37°C for 20 minutes.

Western blot - Anti-LIPC antibody (AB217360)
  • WB

Supplier Data

Western blot - Anti-LIPC antibody (AB217360)

All lanes:

Western blot - Anti-LIPC antibody (ab217360) at 1/300 dilution

All lanes:

HeLa cell lysate

Secondary

All lanes:

Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human LIPC aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIPC also known as Hepatic Lipase plays an important role in lipid metabolism. LIPC has a molecular mass of approximately 56 kDa. It is primarily expressed in the liver and facilitates the hydrolysis of triglycerides and phospholipids in lipoproteins. By acting on lipid substrates LIPC aids in the conversion of intermediate-density lipoproteins (IDL) to low-density lipoproteins (LDL) and helps maintain a balance of plasma lipoproteins.
Biological function summary

The protein contributes to the remodeling of high-density lipoproteins (HDL) and acts in concert with other enzymes to regulate lipid levels in the bloodstream. LIPC functions as a monomer and not as part of a larger complex. Its enzymatic activity impacts the size and composition of HDL particles influencing reverse cholesterol transport. This functional role makes it significant in lipid transport and overall cholesterol homeostasis.

Pathways

LIPC integrates into the lipid and lipoprotein metabolism pathway and the cholesterol biosynthesis pathway. It interacts closely with proteins like Apolipoprotein E (ApoE) and Apolipoprotein C-II (ApoC-II) which modulate its activity and lipid processing capabilities. LIPC's role in converting IDL to LDL connects it to the broader context of lipoprotein metabolism influencing cholesterol transport and clearance mechanisms.

LIPC links to cardiovascular diseases and metabolic syndrome. Abnormal LIPC activity may lead to altered lipoprotein profiles and contribute to atherosclerosis development. Through this context LIPC indirectly associates with the Low-Density Lipoprotein Receptor (LDLR) as both are involved in lipid level regulation and cardiovascular health. Understanding LIPC's implications in these conditions can aid in therapeutic strategies targeting lipid abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins, including chylomicrons, intermediate density lipoproteins (IDL), low density lipoproteins (LDL) of large size and high density lipoproteins (HDL), releasing free fatty acids (FFA) and smaller lipoprotein particles (PubMed : 12032167, PubMed : 26193433, PubMed : 7592706, PubMed : 8798474). Also exhibits lysophospholipase activity (By similarity). Can hydrolyze both neutral lipid and phospholipid substrates but shows a greater binding affinity for neutral lipid substrates than phospholipid substrates (By similarity). In native LDL, preferentially hydrolyzes the phosphatidylcholine species containing polyunsaturated fatty acids at sn-2 position (PubMed : 26193433).
See full target information LIPC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 19:442-448 PubMed31897157

2020

Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Jia,Wenzhong Yang,Xiaohui Zhou,Lu Han,Jumei Shi
View all publications
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Product promise

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