AB124831

Anti-LIPF antibody [EPR5711(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal LIPF antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

Gastric triacylglycerol lipase, GL, Gastric lipase, LIPF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPF antibody [EPR5711(B)] (AB124831)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPF antibody [EPR5711(B)] (AB124831)

ab124831 at 1/250 dilution staining LIPF in paraffin-embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-LIPF antibody [EPR5711(B)] (AB124831)

All lanes:

Western blot - Anti-LIPF antibody [EPR5711(B)] (ab124831) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Mouse small intestine lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 45 kDa

false

Carrier free

Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5711(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "(heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme LIPF also known as gastric lipase plays an essential role in the digestion of dietary fats. It has a molecular mass of approximately 50 kDa. LIPF demonstrates expression mainly in the gastric chief cells within the stomach lining where it catalyzes the hydrolysis of triglycerides into free fatty acids and monoglycerides. This enzymatic activity holds particular importance under acidic pH conditions typically found in the stomach.

Biological function summary

The LIPF enzyme facilitates lipid digestion and absorption in infants and adults. It works independently rather than as part of a larger protein complex. The lipolytic activity of LIPF partially compensates for the less developed pancreatic lipase activity in neonates and provides an early step in lipid breakdown essential for efficient nutrient absorption.

Pathways

The enzyme participates in lipid metabolism and digestive pathways. Within the lipid metabolism pathway LIPF acts similarly with pancreatic lipase but functions specifically in acidic environments highlighting its role in the initial stages of fat digestion. The digestive pathway includes related proteins such as pancreatic lipase which complements LIPF's function by further processing lipids once they enter the duodenum after passing through the stomach.

LIPF influences conditions related to fat malabsorption and gastrointestinal disorders. Its dysfunction or deficiency can lead to conditions like steatorrhea characterized by excess fat in stools due to improper digestion. Furthermore LIPF has connections to Helico pylori infections as this bacterium can affect gastric environments and therefore impact lipid digestion efficiency whereas proteins such as mucin can interact with this process by forming protective barriers for the gastric lining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol (PubMed : 10358049, PubMed : 2243091). Shows a preferential hydrolysis at the sn-3 position of triacylglycerol (PubMed : 2243091).

See full target information LIPF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 50:344-363 PubMed40297641

2025

Multifunctional piezoelectric hydrogels under ultrasound stimulation boost chondrogenesis by recruiting autologous stem cells and activating the Ca/CaM/CaN signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bao Liu,Xu Liu,Xiao-Fei Li,Liang Qiao,Hao-Liang Wang,Yue-Fu Dong,Feng Zhang,Yang Liu,Hao-Yang Liu,Ming-Liang Ji,Lan Li,Qing Jiang,Jun Lu

Cell genomics 3:100420 PubMed38020975

2023

Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Wang,Marta Martínez-Bonet,Taehyeung Kim,Jeffrey A Sparks,Kazuyoshi Ishigaki,Xiaoting Chen,Marc Sudman,Vitor Aguiar,Sangwan Sim,Marcos Chiñas Hernandez,Darren J Chiu,Alexandra Wactor,Brian Wauford,Miranda C Marion,Maria Gutierrez-Arcelus,John Bowes,Stephen Eyre,Ellen Nordal,Sampath Prahalad,Marite Rygg,Vibeke Videm,Soumya Raychaudhuri,Matthew T Weirauch,Carl D Langefeld,Susan D Thompson,Peter A Nigrovic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIPF antibody [EPR5711(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPF antibody [EPR5711(B)] (AB124831)

Western blot - Anti-LIPF antibody [EPR5711(B)] (AB124831)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise