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AB248025

Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LIPF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Gastric triacylglycerol lipase, GL, Gastric lipase, LIPF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free (AB248025)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free (AB248025)

This data was developed using ab124831, the same antibody clone in a different buffer formulation.

ab124831, at 1/250 dilution, staining LIPF in paraffin-embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free (AB248025)
  • WB

Unknown

Western blot - Anti-LIPF antibody [EPR5711(B)] - BSA and Azide free (AB248025)

This data was developed using ab124831, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LIPF antibody [EPR5711(B)] (<a href='/en-us/products/primary-antibodies/lipf-antibody-epr5711b-ab124831'>ab124831</a>) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Mouse small intestine lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5711(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248025 is the carrier-free version of ab124831.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme LIPF also known as gastric lipase plays an essential role in the digestion of dietary fats. It has a molecular mass of approximately 50 kDa. LIPF demonstrates expression mainly in the gastric chief cells within the stomach lining where it catalyzes the hydrolysis of triglycerides into free fatty acids and monoglycerides. This enzymatic activity holds particular importance under acidic pH conditions typically found in the stomach.
Biological function summary

The LIPF enzyme facilitates lipid digestion and absorption in infants and adults. It works independently rather than as part of a larger protein complex. The lipolytic activity of LIPF partially compensates for the less developed pancreatic lipase activity in neonates and provides an early step in lipid breakdown essential for efficient nutrient absorption.

Pathways

The enzyme participates in lipid metabolism and digestive pathways. Within the lipid metabolism pathway LIPF acts similarly with pancreatic lipase but functions specifically in acidic environments highlighting its role in the initial stages of fat digestion. The digestive pathway includes related proteins such as pancreatic lipase which complements LIPF's function by further processing lipids once they enter the duodenum after passing through the stomach.

LIPF influences conditions related to fat malabsorption and gastrointestinal disorders. Its dysfunction or deficiency can lead to conditions like steatorrhea characterized by excess fat in stools due to improper digestion. Furthermore LIPF has connections to Helico pylori infections as this bacterium can affect gastric environments and therefore impact lipid digestion efficiency whereas proteins such as mucin can interact with this process by forming protective barriers for the gastric lining.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol (PubMed : 10358049, PubMed : 2243091). Shows a preferential hydrolysis at the sn-3 position of triacylglycerol (PubMed : 2243091).
See full target information LIPF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You
View all publications
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Product promise

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