Rabbit Recombinant Monoclonal Lipin 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--western-blot-img153771.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118121.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38195.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunocytochemistry-immunofluorescence-img40955.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539)")
![Flow Cytometry (Intracellular) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--flow-cytometry-intracellular-img120743.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (AB247539)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Heat mediated antigen retrieval and use of an HRP/AP polymerized secondary antibody recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:20231281, PubMed:23426360, PubMed:29765047, PubMed:31695197). Is involved in adipocyte differentiation (By similarity). Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression (By similarity).
Alternative names
KIAA0188, LPIN1, Phosphatidate phosphatase LPIN1, Lipin-1
Recommended products
Rabbit Recombinant Monoclonal Lipin 1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3725
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247539 is the carrier-free version of Anti-Lipin 1 antibody [EPR3725] ab92316.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--western-blot-img153771.jpg "Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)")
Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
This data was developed using Anti-Lipin 1 antibody [EPR3725] ab92316, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Lipin 1 antibody [EPR3725] (Anti-Lipin 1 antibody [EPR3725] ab92316) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HuT-78 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 99 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118121.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
This data was developed using Anti-Lipin 1 antibody [EPR3725] ab92316, the same antibody clone in a different buffer formulation.Anti-Lipin 1 antibody [EPR3725] ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38195.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
This data was developed using Anti-Lipin 1 antibody [EPR3725] ab92316, the same antibody clone in a different buffer formulation.Anti-Lipin 1 antibody [EPR3725] ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--immunocytochemistry-immunofluorescence-img40955.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)")
Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
This data was developed using Anti-Lipin 1 antibody [EPR3725] ab92316, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells using Anti-Lipin 1 antibody [EPR3725] ab92316 at 1/100 dilution.
![Flow Cytometry (Intracellular) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539), expandable thumbnail](https://content.abcam.com/products/images/lipin-1-antibody-epr3725-bsa-and-azide-free-ab247539--flow-cytometry-intracellular-img120743.jpg "Flow Cytometry (Intracellular) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)")
Flow Cytometry (Intracellular) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
This data was developed using Anti-Lipin 1 antibody [EPR3725] ab92316, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with Anti-Lipin 1 antibody [EPR3725] ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Lipin 1 antibody [EPR3725] ab92316, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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