Rabbit Recombinant Monoclonal Lipin 2/LPIN2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347), expandable thumbnail](https://content.abcam.com/products/images/lipin-2-lpin2-antibody-epr12479-ab176347--western-blot-img99825.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)")
![Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347), expandable thumbnail](https://content.abcam.com/products/images/lipin-2-lpin2-antibody-epr12479-ab176347--flow-cytometry-intracellular-img50459.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)")
Publications
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- International immunopharmacology 118:1099302023Lipin2 ameliorates diabetic encephalopathy via suppressing JNK/ERK-mediated NLRP3 inflammasome overactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochen Zhang,Shan Huang,Ziyun Zhuang,Xiaolin Han,Min Xie,Shuyan Yu,Mengyu Hua,Zhonghao Liang,Chao Meng,Ling Yin,Xianghua Zhuang,Shihong ChenPubMed 37001383
- Biomolecules & therapeutics 30:246-2562021Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Yi Dou,Yu-Chen Jiang,Zhong-He Hu,Kun-Chen Yao,Ming-Hui Yuan,Xiao-Xue Bao,Mei-Jie Zhou,Yue Liu,Zhao-Xu Li,Li-Hua Lian,Ji-Xing Nan,Yan-Ling WuPubMed 34815367
- Frontiers in pharmacology 12:7386892021L. Extracts Promote Activity of FXR to Ameliorate Alcoholic Liver Disease: Targeting Liver Lipid Deposition and Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Yu Cui,Xin Han,Yu-Chen Jiang,Jia-Yi Dou,Kun-Chen Yao,Zhong-He Hu,Ming-Hui Yuan,Xiao-Xue Bao,Mei-Jie Zhou,Yue Liu,Li-Hua Lian,Xian Zhang,Ji-Xing Nan,Yan-Ling WuPubMed 34690775
- IUBMB life 72:533-5432020Phosphatidic acid metabolism regulates neuroendocrine secretion but is not under the direct control of lipins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire Baneux,Emeline Tanguy,Tamou Thahouly,Alessio Vitale,Sylvette Chasserot-Golaz,Marie-France Bader,Stéphane Gasman,Nicolas VitalePubMed 31967386
- Cell reports 26:884-892.e42019Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongxu Zhao,Zhuanghui Feng,Yongxian Zhang,Yingmin Sun,Yanhao Chen,Xiaojian Liu,Shuang Li,Tingting Zhou,Lanlan Chen,Yuda Wei,Danjun Ma,Kathy O Lui,Hao Ying,Yan Chen,Qiurong DingPubMed 30673611
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Plays important roles in controlling the metabolism of fatty acids at different levels. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.
Alternative names
KIAA0249, LPIN2, Phosphatidate phosphatase LPIN2, Lipin-2
Recommended products
Rabbit Recombinant Monoclonal Lipin 2/LPIN2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12479
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347), expandable thumbnail](https://content.abcam.com/products/images/lipin-2-lpin2-antibody-epr12479-ab176347--western-blot-img99825.jpg "Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)")
Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)
All lanes: Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Predicted band size: 99 kDa
![Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347), expandable thumbnail](https://content.abcam.com/products/images/lipin-2-lpin2-antibody-epr12479-ab176347--flow-cytometry-intracellular-img50459.jpg "Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)")
Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Lipin 2/LPIN2 with ab176347 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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