AB176347

Anti-Lipin 2/LPIN2 antibody [EPR12479]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal Lipin 2/LPIN2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

KIAA0249, LPIN2, Phosphatidate phosphatase LPIN2, Lipin-2

2 Images

Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Lipin 2/LPIN2 with ab176347 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)

All lanes:

Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 99 kDa

false

Carrier free

Anti-Lipin 2/LPIN2 antibody [EPR12479] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12479

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the endoplasmic reticulum membrane. Plays important roles in controlling the metabolism of fatty acids at different levels. Also acts as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.

See full target information LPIN2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37535403

2023

Promoting axon regeneration by inhibiting RNA N6-methyladenosine demethylase ALKBH5.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wang,Tiemei Zheng,Songlin Zhou,Mingwen Liu,Yaobo Liu,Xiaosong Gu,Susu Mao,Bin Yu

International immunopharmacology 118:109930 PubMed37001383

2023

Lipin2 ameliorates diabetic encephalopathy via suppressing JNK/ERK-mediated NLRP3 inflammasome overactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Zhang,Shan Huang,Ziyun Zhuang,Xiaolin Han,Min Xie,Shuyan Yu,Mengyu Hua,Zhonghao Liang,Chao Meng,Ling Yin,Xianghua Zhuang,Shihong Chen

Biomolecules & therapeutics 30:246-256 PubMed34815367

2021

Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Yi Dou,Yu-Chen Jiang,Zhong-He Hu,Kun-Chen Yao,Ming-Hui Yuan,Xiao-Xue Bao,Mei-Jie Zhou,Yue Liu,Zhao-Xu Li,Li-Hua Lian,Ji-Xing Nan,Yan-Ling Wu

Frontiers in pharmacology 12:738689 PubMed34690775

2021

L. Extracts Promote Activity of FXR to Ameliorate Alcoholic Liver Disease: Targeting Liver Lipid Deposition and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Yu Cui,Xin Han,Yu-Chen Jiang,Jia-Yi Dou,Kun-Chen Yao,Zhong-He Hu,Ming-Hui Yuan,Xiao-Xue Bao,Mei-Jie Zhou,Yue Liu,Li-Hua Lian,Xian Zhang,Ji-Xing Nan,Yan-Ling Wu

IUBMB life 72:533-543 PubMed31967386

2020

Phosphatidic acid metabolism regulates neuroendocrine secretion but is not under the direct control of lipins.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Baneux,Emeline Tanguy,Tamou Thahouly,Alessio Vitale,Sylvette Chasserot-Golaz,Marie-France Bader,Stéphane Gasman,Nicolas Vitale

Cell reports 26:884-892.e4 PubMed30673611

2019

Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxu Zhao,Zhuanghui Feng,Yongxian Zhang,Yingmin Sun,Yanhao Chen,Xiaojian Liu,Shuang Li,Tingting Zhou,Lanlan Chen,Yuda Wei,Danjun Ma,Kathy O Lui,Hao Ying,Yan Chen,Qiurong Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lipin 2/LPIN2 antibody [EPR12479]

Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)

Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (AB176347)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise