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Mouse Monoclonal Lipocalin-2 / NGAL antibody. Suitable for WB, IHC-FoFr, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LCN2.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (AB23477), expandable thumbnail
  • Western blot - Anti-Lipocalin-2 / NGAL antibody [5G5] (AB23477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (AB23477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (AB23477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (AB23477), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human LCN2. Database link P80188

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FoFrIHC-P
Human
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:20581821, PubMed:27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368).

Alternative names

HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25

Recommended products

Mouse Monoclonal Lipocalin-2 / NGAL antibody. Suitable for WB, IHC-FoFr, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LCN2.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human LCN2. Database link P80188
Clone number
5G5
Purification technique
Affinity purification Protein G
Specificity

ab23477 is specific for Lipocalin-2 / NGAL, native and denatured forms.

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)

    ab23477 staining human skin tissue sections by IHC-P. Sections were fixed in 10% neutral buffered formalin and subjected to heat mediated antigen retrieval prior to blocking with 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C. A biotinylated horse anti-mouse antibody diluted 1/200 was used as the secondary.

  • Western blot - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477), expandable thumbnail

    Western blot - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)

    Lanes 1 - 4: Merged signal (red and green). Green - ab23477 observed at 23 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

    ab23477 was shown to react with Lipocalin-2 / NGAL in wild-type SW480 cells in Western blot with loss of signal observed in LCN2 knockout knockout cell line Human LCN2 knockout SW480 cell line ab270486 (knockout cell lysate Human LCN2 knockout SW480 cell lysate ab270509). Wild-type SW480 and LCN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab23477 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477) at 1/1000 dilution

    Lane 1: Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: LCN2 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human LCN2 knockout SW480 cell line (Human LCN2 knockout SW480 cell line ab270486)

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human PaCa tissue sections labelling Lipocalin 2 with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human chronic pancreatits tissue sections labelling Lipocalin-2 / NGAL with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human pancreas tissue sections labelling Lipocalin-2 / NGAL with ab23477.

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