Anti-Lipocalin-2 / NGAL antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody (AB41105)

ICC/IF image of ab41105 stained human Hek293 cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41105, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• WB

Lab

Western blot - Anti-Lipocalin-2 / NGAL antibody (AB41105)

All lanes:

Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Lipocalin-2 / NGAL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-lipocalin-2-ngal-protein-ab95007'>ab95007</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 22 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Anti-Lipocalin-2 / NGAL antibody (AB41105)

All lanes:

Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/mL

All lanes:

Human colon tissue lysate - total protein (<a href='/en-us/products/unavailable/human-colon-tissue-lysate-total-protein-ab30051'>ab30051</a>) at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 21 kDa,25 kDa,70 kDa

true

Exposure time: 12min