Rabbit Polyclonal Lipocalin-2 / NGAL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 18 publications.
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Publications
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- International journal of molecular sciences 23:2022Neuroprotection and Neuroregeneration Strategies Using the rNAION Model: Theory, Histology, Problems, Results and Analytical Approaches.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteven L Bernstein,Yan Guo,Zara Mehrabian,Neil R MillerPubMed 36555246
- World journal of gastrointestinal oncology 13:1506-15172021Combination of neutrophil gelatinase-associated lipocalin and matrix metalloproteinase-9 are biomarkers for the detection of colon tubular adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Hua Yuan,Li-Shuang Xie,Yu-Hua Zhu,Xiao-Hua Wang,Yi-Jing Zhang,Xiao-Jun WangPubMed 34721781
- Molecular medicine reports 22:1409-14192020The gonadal expression pattern of lipocalin‑2 and 24p3 receptor is modified in the gonads of the offspring of obese rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElsa De La Chesnaye et. al.PubMed 32627017
- Pediatric research 84:813-8202018Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Ferretti et. al.PubMed 30341414
- Stem cells and development 27:1518-15252018Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYennifer Cortés-Araya,Karin Amilon,Burgunde Elisabeth Rink,Georgina Black,Zofia Lisowski,Francesc Xavier Donadeu,Cristina L EstevesPubMed 30044182
- Cell and tissue research 374:339-3482018Expression of neutrophil gelatinase-associated lipocalin (NGAL) in the gut in Crohn's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilje Thorsvik et. al.PubMed 29869714
- Experimental and therapeutic medicine 16:213-2212018Expression profiling of lipocalin-2 and 24p3 receptor in murine gonads at different developmental stages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElsa De La Chesnaye et. al.PubMed 29896242
- Stem cell research & therapy 8:1572017Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca M Harman et. al.PubMed 28676123
- PloS one 12:e01709512017Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats.
- Critical care (London, England) 20:4062016Blood transfusion improves renal oxygenation and renal function in sepsis-induced acute kidney injury in rats.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
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Target data
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:20581821, PubMed:27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368).
Alternative names
HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25
Recommended products
Rabbit Polyclonal Lipocalin-2 / NGAL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
A customer has submitted a positive Abreview for rat samples in IHC. However, customers have experienced problems with rat samples and this is likely due to the low homology between the immunogen and the rat protein (30%). Some batches of this antibody may work in rat, but this is not batch tested.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105)
All lanes: Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/mL
All lanes: Human colon tissue lysate - total protein (ab30051) at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 21 kDa, 25 kDa, 70 kDa
Exposure time: 12min
Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105)
All lanes: Western blot - Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/mL
All lanes: Western blot - Recombinant Human Lipocalin-2 / NGAL protein (Recombinant Human Lipocalin-2 / NGAL protein ab95007) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Exposure time: 1min
Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody (ab41105)
ICC/IF image of ab41105 stained human Hek293 cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41105, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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