AB216462

Anti-Lipocalin-2 / NGAL antibody [EPR21092]

RabMAb
Recombinant
20ul selling size
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(39 Publications)

Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462) is a rabbit monoclonal antibody detecting Lipocalin-2 / NGAL in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on rat lung (PMID : 27136530; PMID : 27729871) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on mouse lung (PMID : 27136530; PMID : 27729871) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on mouse spleen (PMID : 27729871; PMID : 24587658) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Lipocalin-2 / NGAL with ab216462 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on rat spleen (PMID : 27729871; PMID : 24587658) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile of this antibody is consistent with a recent Nature paper (PMID : 28273060).

All lanes:

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462) at 1/1000 dilution

Lane 1:

Mouse bone tissue lysate at 20 µg

Lane 2:

Mouse white adipose tissue lysate at 20 µg

Lane 3:

Rat bone tissue lysate at 10 µg

Lane 4:

Mouse lung tissue lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 23 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Low expression tissue : heart (PMID : 28615213), brain (PMID : 20860895, PMID : 21184133).

All lanes:

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa

false

Exposure time: 180s

Biotin

Biotin Anti-Lipocalin-2 / NGAL [EPR21092]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Lipocalin-2 / NGAL antibody [EPR21092]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Lipocalin-2 / NGAL antibody [EPR21092]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21092

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not recommended for use in Cell Samples in Western Blot as it detected a strong nonspecific band close to the NGAL position.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "1/2000 dilution recommended for mouse and rat tissues. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "1/2000 dilution recommended for mouse and rat tissues. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Lipocalin-2 / NGAL antibody [EPR21092] (ab216462) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of Lipocalin-2 / NGAL?
Anti-Lipocalin-2 / NGAL [EPR21092] (ab216462) specifically detects a band for Lipocalin-2 / NGAL (UniProt: P11672) at a molecular weight of 23kDa.

Trusted by the scientific community
Anti-Lipocalin-2 / NGAL [EPR21092] (ab216462) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21092] also available for your convenience: ab216462, Biotin - ab318314, Alexa Fluor^® 488 - ab321939, Alexa Fluor^® 647 - ab321940

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).

See full target information Lcn2

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed40573186

2025

Drug Pair of Astragali Radix-Ligustri Lucidi Fructus Alleviates Acute Kidney Injury in Mice Induced by Ischemia-Reperfusion Through Inhibiting Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Liu,Dan Zhang,Yuting Xie,Mengdan Wang,Xiaochun Chen,Weijie Yu,Yuming Ma,Jia Zeng,Qixuan Long,Guangrui Huang,Jie Geng,Anlong Xu

International journal of biological sciences 21:3397-3415 PubMed40520011

2025

Luteolin improves precancerous conditions of the gastric mucosa by binding STAT3 and inhibiting LCN2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Hao,Shouli Yuan,Jing Ning,Yuanfei Zhou,Yanfei Lang,Xiurui Han,Qiao Meng,Ying Xiong,Rongli Cui,Yueqing Gong,Chao Ma,Weichao Xu,Yangang Wang,Xiaohuan Guo,Chu Wang,Jing Zhang,Weiwei Fu,Shigang Ding

Molecular medicine (Cambridge, Mass.) 31:190 PubMed40375133

2025

Lipocalin-2-mediated ferroptosis as a target for protection against light-induced photoreceptor degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Tang,Ruyi Zhai,Jun Ma,Gezhi Xu

Gut microbes 17:2505119 PubMed40364435

2025

Faecalibacterium prausnitzii prevents age-related heart failure by suppressing ferroptosis in cardiomyocytes through butyrate-mediated LCN2 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Ying Wei,Xuejie Han,Ling Shi,Hui Yu,Xuanrui Ji,Yunlong Gao,Qianhui Gao,Linwei Zhang,Yu Duan,Wenpeng Li,Yue Yuan,Jing Shi,Liang Cheng,Yue Li

Theranostics 15:4763-4784 PubMed40225581

2025

Microglial Lcn2 knockout enhances chronic intracerebral hemorrhage recovery by restoring myelin and reducing inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Lei Zhang,Kai Wang,Jun He,Liang Yuan,Yangang Wang,Weihao Lv,Zehan Zhang,Yuan Feng,Hongchen Zhang,Min Zhang,Rui Lv,Ya-Nan Dou,Xiaowei Fei,Jialiang Wei

Immunity, inflammation and disease 13:e70170 PubMed40084946

2025

Ferroptosis-Related Genes Are Effective Markers for Diagnostic Targets of Crohn's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Liu,Qing Liu,Ye Tian,Pengpeng Cai,Jianan Bai

International journal of medical sciences 22:971-981 PubMed39991763

2025

Kidney Injury under Diminished Pulsatile Flow Induced by V-A ECMO in Sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Yan,Tianlong Wang,Jiachen Qi,Luyu Bian,Shengqiang Pei,Qiaoni Zhang,Sizhe Gao,Shujie Yan,Gang Liu,Yuan Teng,Jian Wang,Chun Zhou,Qian Wang,Jing Wang,Bingyang Ji

Frontiers in cellular and infection microbiology 14:1507914 PubMed39963236

2025

Attenuation of acute kidney injury in a murine model of neonatal sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Esther M Speer,Atilade A Adedeji,Joyce Lin,Alexandra Khorasanchi,Asma Rasheed,Maya Bhat,Kelly Mackenzie,Randolph Hennigar,Kimberly J Reidy,Robert P Woroniecki

American journal of physiology. Cell physiology 328:C699-C709 PubMed39932439

2025

Rab7-regulated ferroptosis contributes to tubular epithelial cells injury by degradation of GPX4 via chaperone-mediated autophagy in AKI.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Qishuang Wei,Renyu Wang,Hui Sun,Sijing He,Lijuan Tang,Shuang Zhang,Yifei Liu,Shali Yu

Biomolecules 14: PubMed39766229

2025

Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion?

Applications

Unspecified application

Species

Unspecified reactive species

You Hyun Jeon,Eun-Joo Oh,Se-Hyun Oh,Jeong-Hoon Lim,Hee-Yeon Jung,Ji-Young Choi,Jang-Hee Cho,Sun-Hee Park,Yong-Lim Kim,Chan-Duck Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lipocalin-2 / NGAL antibody [EPR21092]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR21092] (AB216462)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Properties and storage information

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Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (39)

Product promise