AB208184

Anti-Lipoprotein a antibody [EPR19731]

RabMAb
Recombinant
What is this?

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(2 Publications)

Anti-Lipoprotein a antibody [EPR19731] (ab208184) is a rabbit monoclonal antibody detecting Lipoprotein a in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Apolipoprotein(a), Apo(a), Lp(a), LPA, AK38, Antiangiogenic AK38 protein, apo(a), APOA, apolipoprotein(a), LP, lp(a), LPA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on blood vessels of human glioma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9590382, 10484779 and 10703674).

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Lane 3:

Human liver tissue lysate at 10 µg

Lane 4:

Human blood at 10 µg

Lane 5:

Human adipose tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 501 kDa

Observed band size: 100-500 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9590382, 10484779 and 10703674).

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Lane 3:

Human liver lysate at 10 µg

Lane 4:

Human blood at 10 µg

Lane 5:

Human adipose lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 501 kDa

Observed band size: 100-500 kDa

false

Exposure time: 30s

Carrier free

Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19731

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Lipoprotein a antibody [EPR19731] (ab208184) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Lipoprotein a?
Anti-Lipoprotein a [EPR19731] (ab208184) specifically detects a band for Lipoprotein a (UniProt: P08519) at a molecular weight of 501kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19731] also available for your convenience: ab208184, Carrier free - ab251491

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.

See full target information LPA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40889156

2025

SMAD3 and p300 complex scaffolding by long non-coding RNA LIMD1-AS1 promotes TGF-β-induced breast cancer cell plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Chuannan Fan,Davy Cats,Miriam Selle,Olga Khorosjutina,Soniya Dhanjal,Bernhard Schmierer,Hailiang Mei,Peter Ten Dijke,Qian Wang

Arteriosclerosis, thrombosis, and vascular biology 43:1851-1866 PubMed37589135

2023

Plasminogen Receptors Promote Lipoprotein(a) Uptake by Enhancing Surface Binding and Facilitating Macropinocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Halima Siddiqui,Nikita Deo,Malcolm T Rutledge,Michael J A Williams,Gregory M I Redpath,Sally P A McCormick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lipoprotein a antibody [EPR19731]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

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Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise