Anti-Lipoprotein a antibody [EPR19731] (ab208184) is a rabbit monoclonal antibody that is used to detect Lipoprotein a in Western Blot, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--western-blot-img9400.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)")
![Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--western-blot-img7384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein a antibody [EPR19731] (AB208184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128535.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
Additional Targets
Lipoprotein a
Alternative names
Apolipoprotein(a), Apo(a), Lp(a), LPA
Recommended products
Anti-Lipoprotein a antibody [EPR19731] (ab208184) is a rabbit monoclonal antibody that is used to detect Lipoprotein a in Western Blot, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19731
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Lipoprotein a antibody [EPR19731] (ab208184) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human samples.
What is the molecular weight of Lipoprotein a?
Anti-Lipoprotein a [EPR19731] (ab208184) specifically detects a band for Lipoprotein a (UniProt: P08519) at a molecular weight of 501kDa.
Recommended positive controls
WB: Human serum, plasma, liver, blood and adipose lysates.IHC-P: Human liver and glioma tissues.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR19731] also available for your convenience:
ab208184, Carrier free - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free ab251491
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--western-blot-img9400.jpg "Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)")
Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).
All lanes: Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution
Lane 1: Human serum at 10 µg
Lane 2: Human plasma at 10 µg
Lane 3: Human liver tissue lysate at 10 µg
Lane 4: Human blood at 10 µg
Lane 5: Human adipose tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 501 kDa
Observed band size: 100-500 kDa
Exposure time: 3min
![Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--western-blot-img7384.jpg "Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)")
Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9590382, 10484779 and 10703674).
All lanes: Western blot - Anti-Lipoprotein a antibody [EPR19731] (ab208184) at 1/5000 dilution
Lane 1: Human serum at 10 µg
Lane 2: Human plasma at 10 µg
Lane 3: Human liver lysate at 10 µg
Lane 4: Human blood at 10 µg
Lane 5: Human adipose lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 501 kDa
Observed band size: 100-500 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128535.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr19731-ab208184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (ab208184)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on blood vessels of human glioma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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