Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (AB251491)

This data was developed using ab208184, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (AB251491)

This data was developed using ab208184, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Lipoprotein a with ab208184 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on blood vessels of human glioma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (AB251491)

This data was developed using ab208184, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9590382, 10484779 and 10703674).

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR19731] (<a href='/en-us/products/primary-antibodies/lipoprotein-a-antibody-epr19731-ab208184'>ab208184</a>) at 1/5000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Lane 3:

Human liver tissue lysate at 10 µg

Lane 4:

Human blood at 10 µg

Lane 5:

Human adipose tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 501 kDa

Observed band size: 100-500 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Lipoprotein a antibody [EPR19731] - BSA and Azide free (AB251491)

This data was developed using ab208184, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9590382, 10484779 and 10703674).

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR19731] (<a href='/en-us/products/primary-antibodies/lipoprotein-a-antibody-epr19731-ab208184'>ab208184</a>) at 1/5000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Lane 3:

Human liver lysate at 10 µg

Lane 4:

Human blood at 10 µg

Lane 5:

Human adipose lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 501 kDa

Observed band size: 100-500 kDa

false

Exposure time: 30s