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AB125014

Anti-Lipoprotein a antibody [EPR6474]

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(4 Publications)

Anti-Lipoprotein a antibody [EPR6474] (ab125014) is a rabbit monoclonal antibody detecting Lipoprotein a in Western Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Apolipoprotein(a), Apo(a), Lp(a), LPA

2 Images
Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)
  • WB

Lab

Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)

Blocking and dilution buffer : 5% NFDM /TBST.

The observed bands are consistant with desciption in PMID : 10703674, which should be N-terminal fragments.

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Predicted band size: 501 kDa

Observed band size: 501 kDa

false

Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)
  • WB

Unknown

Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)

All lanes:

Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 501 kDa

Observed band size: 500 kDa

false

  • Carrier free

    Anti-Lipoprotein a antibody [EPR6474] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6474

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Anti-Lipoprotein a antibody [EPR6474] (ab125014) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Lipoprotein a?
Anti-Lipoprotein a [EPR6474] (ab125014) specifically detects a band for Lipoprotein a (UniProt: P08519) at a molecular weight of 501kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR6474] also available for your convenience: ab125014, Carrier free - ab240014

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
See full target information LPA

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:9964689 PubMed35096275

2022

Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory 7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery Spasm.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Kuang Lin,Chi-Tai Yeh,Kuang-Tai Kuo,Iat-Hang Fong,Vijesh Kumar Yadav,Nicholas G Kounis,Patrick Hu,Ming-Yow Hung

Hepatology (Baltimore, Md.) 65:1851-1864 PubMed28152568

2017

Apolipoprotein(a) inhibits hepatitis C virus entry through interaction with infectious particles.

Applications

Unspecified application

Species

Unspecified reactive species

Catarina Oliveira,Carole Fournier,Véronique Descamps,Virginie Morel,Corey A Scipione,Rocco Romagnuolo,Marlys L Koschinsky,Agnès Boullier,Paulo Marcelo,Jean-Marc Domon,Etienne Brochot,Gilles Duverlie,Catherine Francois,Sandrine Castelain,Francois Helle

Journal of lipid research 57:1086-96 PubMed27102113

2016

PCSK9 inhibition-mediated reduction in Lp(a) with evolocumab: an analysis of 10 clinical trials and the LDL receptor's role.

Applications

Unspecified application

Species

Unspecified reactive species

Frederick J Raal,Robert P Giugliano,Marc S Sabatine,Michael J Koren,Dirk Blom,Nabil G Seidah,Narimon Honarpour,Armando Lira,Allen Xue,Padmaja Chiruvolu,Simon Jackson,Mei Di,Matthew Peach,Ransi Somaratne,Scott M Wasserman,Rob Scott,Evan A Stein

Journal of lipid research 56:1034-42 PubMed25713100

2015

IL-6 blockade by monoclonal antibodies inhibits apolipoprotein (a) expression and lipoprotein (a) synthesis in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Nike Müller,Dominik M Schulte,Kathrin Türk,Sandra Freitag-Wolf,Jochen Hampe,Rainald Zeuner,Johann O Schröder,Ioanna Gouni-Berthold,Heiner K Berthold,Wilhelm Krone,Stefan Rose-John,Stefan Schreiber,Matthias Laudes
View all publications

Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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