Rabbit Recombinant Monoclonal Lipoprotein a antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr6474-ab125014--western-blot-img7729.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)")
![Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr6474-ab125014--western-blot-img93764.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein a antibody [EPR6474] (AB125014)")
Publications
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- Oxidative medicine and cellular longevity 2022:99646892022Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory 7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery Spasm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen-Kuang Lin,Chi-Tai Yeh,Kuang-Tai Kuo,Iat-Hang Fong,Vijesh Kumar Yadav,Nicholas G Kounis,Patrick Hu,Ming-Yow HungPubMed 35096275
- Hepatology (Baltimore, Md.) 65:1851-18642017Apolipoprotein(a) inhibits hepatitis C virus entry through interaction with infectious particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatarina Oliveira et. al.PubMed 28152568
- Journal of lipid research 57:1086-962016PCSK9 inhibition-mediated reduction in Lp(a) with evolocumab: an analysis of 10 clinical trials and the LDL receptor's role.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrederick J Raal et. al.PubMed 27102113
- Journal of lipid research 56:1034-422015IL-6 blockade by monoclonal antibodies inhibits apolipoprotein (a) expression and lipoprotein (a) synthesis in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNike Müller et. al.PubMed 25713100
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
Alternative names
Apolipoprotein(a), Apo(a), Lp(a), LPA
Recommended products
Rabbit Recombinant Monoclonal Lipoprotein a antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR6474
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr6474-ab125014--western-blot-img7729.jpg "Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)")
Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
Blocking and dilution buffer: 5% NFDM /TBST.
The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.
All lanes: Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution
All lanes: Human plasma lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 501 kDa
Observed band size: 501 kDa
![Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-a-antibody-epr6474-ab125014--western-blot-img93764.jpg "Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)")
Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
All lanes: Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution
All lanes: Human plasma lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 501 kDa
Observed band size: 500 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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