Anti-Lipoprotein lipase antibody [5D2]

3 product images

• 

  Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (ab93898)

  Overlay histogram showing HeLa cells stained with ab93898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93898, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (ab93898)

  ab93898 staining Lipoprotein lipase in wild-type HeLa cells (top panel) and LPL knockout HeLa cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab93898 at 5μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (ab93898)

  Immunocytochemistry/Immunofluorescence analysis of mouse cortical glia labelling Lipoprotein lipase (green) with ab93898 at 2μg/ml. Cells were permemabilized with PBS + 0.1% Triton X-100 and blocked with PBS + 10% FBS. Nuclei stained with Hoechst (blue).