AB93898

Anti-Lipoprotein lipase antibody [5D2]

Name: Anti-Lipoprotein lipase antibody [5D2] (ab93898) | Abcam
Brand: Abcam Limited
SKU: AB93898
Rating: 4 (2 reviews)

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(2 Reviews)

(10 Publications)

Mouse Monoclonal Lipoprotein lipase antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse, Cat samples. Cited in 10 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow LPL.

View Alternative Names

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

ab93898 staining Lipoprotein lipase in wild-type HeLa cells (top panel) and LPL knockout HeLa cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab93898 at 5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Flow Cyt

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Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Overlay histogram showing HeLa cells stained with ab93898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93898, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Immunocytochemistry/Immunofluorescence analysis of mouse cortical glia labelling Lipoprotein lipase (green) with ab93898 at 2μg/ml. Cells were permemabilized with PBS + 0.1% Triton X-100 and blocked with PBS + 10% FBS. Nuclei stained with Hoechst (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5D2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Cat, Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Cow LPL.

P11151

Specificity

This antibody does not recognise hepatic lipase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-5 µg/mL", "ICCIF-species-notes": "" }, "Cat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "2-5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

See full target information LPL

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:12231-12251 PubMed40382530

2025

High-Fat Diet-Induced Excessive Accumulation of Cerebral Cholesterol Esters and Microglial Dysfunction Exacerbate Alzheimer's Disease Pathology in APP mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhan Yang,Hirofumi Miyazaki,Tunyanat Wannakul,Eiko Amo,Takaomi Saido,Takashi Saito,Hiroki Sasaguri,Motoko Maekawa,Yuji Owada

Nature communications 14:2569 PubMed37142573

2023

Structure of dimeric lipoprotein lipase reveals a pore adjacent to the active site.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn H Gunn,Saskia B Neher

Human molecular genetics 32:1466-1482 PubMed36519761

2022

Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Willcyn Tang,John Thundyil,Grace Gui Yin Lim,Teddy J W Tng,Sean Qing Zhang Yeow,Aditya Nair,Chou Chai,Tso-Pang Yao,Kah-Leong Lim

Evidence-based complementary and alternative medicine : eCAM 2022:4463108 PubMed36285165

2022

Danlou Tablet May Alleviate Vascular Injury Caused by Chronic Intermittent Hypoxia through Regulating FIH-1, HIF-1, and Angptl4.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Rong,Qian Wu,Jingjing Tang,Zhiguo Liu,Qianyu Lv,Xuejiao Ye,Yu Dong,Yuebo Zhang,Guangxi Li,Shihan Wang

International journal of molecular sciences 22: PubMed34638947

2021

Leptin Reduces Plin5 mA Methylation through FTO to Regulate Lipolysis in Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Dongqin Wei,Qian Sun,Yizhou Li,Chaowei Li,Xinjian Li,Chao Sun

Molecular neurodegeneration 16:45 PubMed34215298

2021

Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.

Applications

Unspecified application

Species

Unspecified reactive species

Md Golam Sharoar,Sarah Palko,Yingying Ge,Takaomi C Saido,Riqiang Yan

Yonsei medical journal 60:1187-1194 PubMed31769250

2019

MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Wang,Lixin Lin,Yong Huang,Junjun Sun,Xueming Wang,Peng Wang

Molecular nutrition & food research :e1801385 PubMed31004461

2019

Resveratrol Treatment Ameliorates Leptin Resistance and Adiposity Programed by the Combined Effect of Maternal and Post-Weaning High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ren Yu,Jiunn-Ming Sheen,Mao-Meng Tiao,You-Lin Tain,Chih-Cheng Chen,I-Chun Lin,Yun-Ju Lai,Ching-Chou Tsai,Yu-Ju Lin,Ching-Chang Tsai,Kow-Aung Chang,Li-Tung Huang

American journal of physiology. Lung cellular and 309:L435-40 PubMed26115672

2015

Severe pulmonary hypertension is associated with altered right ventricle metabolic substrate uptake.

Applications

IHC-P, WB

Species

Rat, Rat

Brian B Graham,Rahul Kumar,Claudia Mickael,Linda Sanders,Liya Gebreab,Kendra M Huber,Mario Perez,Peter Smith-Jones,Natalie J Serkova,Rubin M Tuder

Journal of lipid research 39:2350-9 PubMed9831623

1998

Detailed characterization of the binding site of the lipoprotein lipase-specific monoclonal antibody 5D2.

Applications

Unspecified application

Species

Unspecified reactive species

S F Chang,B Reich,J D Brunzell,H Will

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Lipoprotein lipase antibody [5D2]

Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Flow Cytometry - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody [5D2] (AB93898)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

Product promise