Rabbit Recombinant Monoclonal Lipoprotein lipase antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (AB316009), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-lipase-antibody-epr28301-66-bsa-and-azide-free-ab316009--western-blot-img426207.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (AB316009)")
![Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (AB316009), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-lipase-antibody-epr28301-66-bsa-and-azide-free-ab316009--western-blot-img426208.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (AB316009)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed:8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (By similarity). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed:24726386). Recruited to its site of action on vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed:20620994, PubMed:24726386, PubMed:27811232).
Alternative names
Lipoprotein lipase, LPL, Phospholipase A1, rep, 1a-1b
Recommended products
Rabbit Recombinant Monoclonal Lipoprotein lipase antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28301-66
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316009 is the carrier-free version of Anti-Lipoprotein lipase antibody [EPR28301-66] ab316008.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-lipase-antibody-epr28301-66-bsa-and-azide-free-ab316009--western-blot-img426207.jpg "Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009)")
Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009)
This data was developed using Anti-Lipoprotein lipase antibody [EPR28301-66] ab316008, the same antibody clone in a different buffer formulation.
Low expression: spleen (PMID: 9727057), liver (PMID: 9727057, 2723548).
The identity of the bands between 25 kDa and 37 kDa (in lane 4) are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] (Anti-Lipoprotein lipase antibody [EPR28301-66] ab316008) at 1/1000 dilution
Lane 1: Mouse brown fat tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 53 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009), expandable thumbnail](https://content.abcam.com/products/images/lipoprotein-lipase-antibody-epr28301-66-bsa-and-azide-free-ab316009--western-blot-img426208.jpg "Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009)")
Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] - BSA and Azide free (ab316009)
This data was developed using Anti-Lipoprotein lipase antibody [EPR28301-66] ab316008, the same antibody clone in a different buffer formulation.
Low expression: Neuro-2a.
The identity of the band at 140 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Lipoprotein lipase antibody [EPR28301-66] (Anti-Lipoprotein lipase antibody [EPR28301-66] ab316008) at 1/1000 dilution
Lane 1: L929 (mouse connective tissue fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 53 kDa, 36 kDa
Exposure time: 92s
Downloads
Product protocols
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