AB21356

Anti-Lipoprotein lipase antibody [LPL.A4]

Name: Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) | Abcam
Brand: Abcam Limited
SKU: AB21356
Price: 480 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(64 Publications)

Mouse Monoclonal Lipoprotein lipase antibody. Suitable for Flow Cyt, WB and reacts with Human, Cow samples. Cited in 64 publications. Immunogen corresponding to Full Length Protein corresponding to Human LPL.

View Alternative Names

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

2 Images

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Flow Cyt

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Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

All lanes:

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

Lane 1:

Human Lipoprotein lipase. at 10 µg

Lane 2:

Bovine Lipoprotein lipase at 10 µg

Predicted band size: 53 kDa

Observed band size: 55 kDa,60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LPL.A4

Isotype

IgG1

Carrier free

Reacts with

Cow, Human

Applications

Flow Cyt, WB

applications

Immunogen

Full Length Protein corresponding to Human LPL. The exact immunogen used to generate this antibody is proprietary information.

P06858

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Protein A affinity chromatography

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

See full target information LPL

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Publications (64)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400064 PubMed38981007

2024

Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Chan Park,Jong Won Han,Woochan Lee,Jieun Kim,Sang-Eun Lee,Dongjoon Lee,Hayoung Choi,Jihui Han,You Jung Kang,Yen N Diep,Hansang Cho,Rian Kang,Won Jong Yu,Jean Lee,Murim Choi,Sun-Wha Im,Jong-Il Kim,Inhee Mook-Jung

Immunology 170:28-46 PubMed37094816

2023

Deficiency of angiopoietin-like 4 enhances CD8 T cell bioactivity via metabolic reprogramming for impairing tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Zhijie Lin,Xiaoyuan Zhang,Xiaoqing Jia,Hualing Li,Yi Fu,Xuefeng Wang,Guoqiang Zhu,Guotao Lu,Weiming Xiao,Weijuan Gong

Frontiers in pharmacology 14:1148737 PubMed37077819

2023

Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis regulation of gut microbiota and bile acid signaling in a mouse model .

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Xin Xin,Qin Feng,Yiyang Hu

Alzheimer's research & therapy 15:53 PubMed36918976

2023

Attenuation of Alzheimer's brain pathology in 5XFAD mice by PTH, a peptide of parathyroid hormone.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Lei Xiong,Lingling Yao,Jinxiu Pan,Emily Arzola,Xiaojuan Zhu,Lin Mei,Wen-Cheng Xiong

Molecular neurodegeneration 17:83 PubMed36536457

2022

Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Won Kim,Kevin J Tu,Alice Wei,Ashley J Lau,Anabel Gonzalez-Gil,Tianyu Cao,Kerstin Braunstein,Jonathan P Ling,Juan C Troncoso,Philip C Wong,Seth Blackshaw,Ronald L Schnaar,Tong Li

Human molecular genetics 32:1466-1482 PubMed36519761

2022

Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Willcyn Tang,John Thundyil,Grace Gui Yin Lim,Teddy J W Tng,Sean Qing Zhang Yeow,Aditya Nair,Chou Chai,Tso-Pang Yao,Kah-Leong Lim

Frontiers in nutrition 9:914079 PubMed36091227

2022

Gypenosides ameliorate high-fat diet-induced non-alcoholic steatohepatitis farnesoid X receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Hongliang Liu,Xin Xin

Frontiers in pharmacology 13:848957 PubMed35652039

2022

Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4 T Cell Activities Direct Binding With Cytochrome P450 1B1.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Guotao Lu,Biying Wang,Jie Xiang,Chunxia Hu,Zhijie Lin,Yanbing Ding,Weiming Xiao,Weijuan Gong

Journal of neuroinflammation 19:61 PubMed35236374

2022

Microglial VPS35 deficiency impairs Aβ phagocytosis and Aβ-induced disease-associated microglia, and enhances Aβ associated pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ren,Lingling Yao,YongGang Wang,Lin Mei,Wen-Cheng Xiong

Nature communications 13:945 PubMed35177618

2022

Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Beuker,David Schafflick,Jan-Kolja Strecker,Michael Heming,Xiaolin Li,Jolien Wolbert,Antje Schmidt-Pogoda,Christian Thomas,Tanja Kuhlmann,Irene Aranda-Pardos,Noelia A-Gonzalez,Praveen Ashok Kumar,Yves Werner,Ertugrul Kilic,Dirk M Hermann,Heinz Wiendl,Ralf Stumm,Gerd Meyer Zu Hörste,Jens Minnerup

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lipoprotein lipase antibody [LPL.A4]

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (64)

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