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AB2607

Anti-LIS1 antibody

2

(2 Reviews)

|

(21 Publications)

Rabbit Polyclonal LIS1 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human PAFAH1B1.

View Alternative Names

LIS1, MDCR, MDS, PAFAHA, PAFAH1B1, Platelet-activating factor acetylhydrolase IB subunit beta, Lissencephaly-1 protein, PAF acetylhydrolase 45 kDa subunit, PAF-AH alpha, LIS-1, PAF-AH 45 kDa subunit, PAFAH alpha

1 Images
Western blot - Anti-LIS1 antibody (AB2607)
  • WB

Supplier Data

Western blot - Anti-LIS1 antibody (AB2607)

All lanes:

Western blot - Anti-LIS1 antibody (ab2607) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

C6 (rat glioma cell line) whole cell lysate at 50 µg

Predicted band size: 47 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PAFAH1B1. The exact immunogen used to generate this antibody is proprietary information.

P43034

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Reactivity data

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Properties and storage information

Form
Liquid
Purification notes
Affinity purified using the immunising peptide immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIS1 also known as PAFAH1B1 is a protein with a molecular mass of approximately 45 kDa. It plays a significant role in cellular and developmental processes by stabilizing microtubules and facilitating intracellular transport. LIS1 expresses ubiquitously with notable presence in the brain and other neural tissues. It interacts with dynein motor complexes guiding the transport of cellular components along microtubules and is essential in mitosis and neuronal migration.
Biological function summary

LIS1 influences neuronal migration during brain development. It forms a complex with dynein and other proteins like NDEL1 which is important for distributing organelles and proteins in the neuron. This dynein-LIS1 complex ensures proper positioning of the nucleus during cell division and migration. It also regulates the alignment of chromosomes warranting correct cytoskeleton organization and cell polarity.

Pathways

LIS1 is deeply involved in neurodevelopmental pathways and the stabilization of the cytoskeleton. The protein is a central player in the Reelin signaling pathway which is essential for layering neurons during cortical development. LIS1 cooperates with other components such as the DISC1 and DCX to ensure proper functioning within these pathways. These interactions are vital for maintaining proper brain structure and neural circuitry.

LIS1 mutations are linked to lissencephaly a disorder characterized by abnormal neuronal migration resulting in a smooth brain structure. This condition stems from disruptions in the mechanisms regulating microtubule dynamics and nuclear positioning during neuronal development. The protein interacts with TUBA1A another protein implicated in lissencephaly affecting the stability and function of microtubules. Disorders such as Miller-Dieker syndrome also relate to abnormalities in LIS1 highlighting its critical role in neurodevelopment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition-dependent manner (By similarity). Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (PubMed : 22956769). May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR (By similarity).
See full target information PAFAH1B1
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 61: PubMed36102310

2022

LIS1 interacts with CLIP170 to promote tumor growth and metastasis via the Cdc42 signaling pathway in salivary gland adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Li,Zhihao Wen,Ni Kou,Jing Liu,Dong Jin,Lina Wang,Fu Wang,Lu Gao

Molecular cell 82:2604-2617.e8 PubMed35654044

2022

A pro-metastatic tRNA fragment drives Nucleolin oligomerization and stabilization of its bound metabolic mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Xuhang Liu,Wenbin Mei,Veena Padmanaban,Hanan Alwaseem,Henrik Molina,Maria C Passarelli,Bernardo Tavora,Sohail F Tavazoie

The Journal of biological chemistry 298:101983 PubMed35483451

2022

microRNA-27b regulates hepatic lipase enzyme LIPC and reduces triglyceride degradation during hepatitis C virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Geneviève F Desrochers,Roxana Filip,Micheal Bastianelli,Tiffany Stern,John Paul Pezacki

The Journal of pathology 254:92-102 PubMed33565082

2021

Human cytomegalovirus infection is associated with increased expression of the lissencephaly gene PAFAH1B1 encoding LIS1 in neural stem cells and congenitally infected brains.

Applications

Unspecified application

Species

Unspecified reactive species

Maude Rolland,Hélène Martin,Mathilde Bergamelli,Yann Sellier,Bettina Bessières,Jacqueline Aziza,Alexandra Benchoua,Marianne Leruez-Ville,Daniel Gonzalez-Dunia,Stéphane Chavanas

Cerebral cortex communications 2:tgaa096 PubMed33615226

2021

Behavioral Deficits in Mice with Postnatal Disruption of in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Cezar Gavrilovici,Yulan Jiang,Ivana Kiroski,Toni-Lee Sterley,Milene Vandal,Jaideep Bains,Sang Ki Park,Jong M Rho,G Campbell Teskey,Minh Dang Nguyen

Cytoskeleton (Hoboken, N.J.) 77:249-260 PubMed32524725

2020

Lis1 co-localizes with actin in the phagocytic cup and regulates phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Aditya Chhatre,Paulomi Sanghavi,Roop Mallik

eLife 9: PubMed32159512

2020

LIS1 determines cleavage plane positioning by regulating actomyosin-mediated cell membrane contractility.

Applications

Unspecified application

Species

Unspecified reactive species

Hyang Mi Moon,Simon Hippenmeyer,Liqun Luo,Anthony Wynshaw-Boris

Annals of neurology 86:99-115 PubMed31004438

2019

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyowon Hong,Kwangsic Joo,Sang Min Park,Jimyung Seo,Min Hwan Kim,EunBie Shin,Hae Il Cheong,Jeong Ho Lee,Joon Kim

Journal of Cancer 8:1284-1291 PubMed28607604

2017

Preferential Association of Lissencephaly-1 Gene Expression with CD133+ Glioblastoma Cells.

Applications

IHC-P

Species

Human

Felix Mircea Brehar,Anca Violeta Gafencu,Violeta Georgeta Trusca,Elena Valeria Fuior,Dorel Arsene,Mirela Amaireh,Andrei Giovani,Mircea Radu Gorgan

PloS one 8:e80605 PubMed24244700

2013

Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes.

Applications

WB

Species

Drosophila melanogaster

Paulomi Sanghavi,Shobha Laxani,Xuan Li,Simon L Bullock,Graydon B Gonsalvez
View all publications
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