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Rabbit Recombinant Monoclonal LIS1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915), expandable thumbnail
  • Western blot - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition-dependent manner (By similarity). Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (PubMed:22956769). May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR (By similarity).

Alternative names

LIS1, MDCR, MDS, PAFAHA, PAFAH1B1, Platelet-activating factor acetylhydrolase IB subunit beta, Lissencephaly-1 protein, PAF acetylhydrolase 45 kDa subunit, PAF-AH alpha, LIS-1, PAF-AH 45 kDa subunit, PAFAH alpha

Recommended products

Rabbit Recombinant Monoclonal LIS1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3335(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247915 is the carrier-free version of Anti-LIS1 antibody [EPR3335(2)] ab109630.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LIS1 also known as PAFAH1B1 is a protein with a molecular mass of approximately 45 kDa. It plays a significant role in cellular and developmental processes by stabilizing microtubules and facilitating intracellular transport. LIS1 expresses ubiquitously with notable presence in the brain and other neural tissues. It interacts with dynein motor complexes guiding the transport of cellular components along microtubules and is essential in mitosis and neuronal migration.

Biological function summary

LIS1 influences neuronal migration during brain development. It forms a complex with dynein and other proteins like NDEL1 which is important for distributing organelles and proteins in the neuron. This dynein-LIS1 complex ensures proper positioning of the nucleus during cell division and migration. It also regulates the alignment of chromosomes warranting correct cytoskeleton organization and cell polarity.

Pathways

LIS1 is deeply involved in neurodevelopmental pathways and the stabilization of the cytoskeleton. The protein is a central player in the Reelin signaling pathway which is essential for layering neurons during cortical development. LIS1 cooperates with other components such as the DISC1 and DCX to ensure proper functioning within these pathways. These interactions are vital for maintaining proper brain structure and neural circuitry.

Associated diseases and disorders

LIS1 mutations are linked to lissencephaly a disorder characterized by abnormal neuronal migration resulting in a smooth brain structure. This condition stems from disruptions in the mechanisms regulating microtubule dynamics and nuclear positioning during neuronal development. The protein interacts with TUBA1A another protein implicated in lissencephaly affecting the stability and function of microtubules. Disorders such as Miller-Dieker syndrome also relate to abnormalities in LIS1 highlighting its critical role in neurodevelopment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

    This data was developed using Anti-LIS1 antibody [EPR3335(2)] ab109630, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with Anti-LIS1 antibody [EPR3335(2)] ab109630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-LIS1 antibody [EPR3335(2)] ab109630, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915), expandable thumbnail

    Western blot - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

    This data was developed using Anti-LIS1 antibody [EPR3335(2)] ab109630, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-LIS1 antibody [EPR3335(2)] (Anti-LIS1 antibody [EPR3335(2)] ab109630) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: SH-SY5Y cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Predicted band size: 47 kDa

  • Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

    This data was developed using Anti-LIS1 antibody [EPR3335(2)] ab109630, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using Anti-LIS1 antibody [EPR3335(2)] ab109630 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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