Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915)

This data was developed using ab109630, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using ab109630 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915)

This data was developed using ab109630, the same antibody clone in a different buffer formulation.

Overlay histogram showing SHSY-5Y cells stained with ab109630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109630, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (AB247915)

This data was developed using ab109630, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LIS1 antibody [EPR3335(2)] (<a href='/en-us/products/primary-antibodies/lis1-antibody-epr33352-ab109630'>ab109630</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 47 kDa

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