Rabbit Recombinant Monoclonal LIS1 antibody. Suitable for WB and reacts with Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human PAFAH1B1.
Images
![Western blot - Anti-LIS1 Antibody [S03-2I0] (AB317390), expandable thumbnail](https://content.abcam.com/products/images/lis1-antibody-s03-2i0-ab317390--western-blot-img429474.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LIS1 Antibody [S03-2I0] (AB317390)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human PAFAH1B1. Database link P43034
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Rat | Tested |
| Chinese hamster | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Chinese hamster | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition-dependent manner (By similarity). Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (By similarity). May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR (By similarity).
Additional Targets
Pafah1b1
Alternative names
Lis-1, Lis1, Pafaha, Platelet-activating factor acetylhydrolase IB subunit alpha, Lissencephaly-1 protein, PAF acetylhydrolase 45 kDa subunit, PAF-AH alpha, LIS-1, PAF-AH 45 kDa subunit, PAFAH alpha
Recommended products
Rabbit Recombinant Monoclonal LIS1 antibody. Suitable for WB and reacts with Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human PAFAH1B1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human PAFAH1B1. Database link P43034
- Clone number
- S03-2I0
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LIS1 also known as PAFAH1B1 is a protein with a molecular mass of approximately 45 kDa. It plays a significant role in cellular and developmental processes by stabilizing microtubules and facilitating intracellular transport. LIS1 expresses ubiquitously with notable presence in the brain and other neural tissues. It interacts with dynein motor complexes guiding the transport of cellular components along microtubules and is essential in mitosis and neuronal migration.
- Biological function summary
LIS1 influences neuronal migration during brain development. It forms a complex with dynein and other proteins like NDEL1 which is important for distributing organelles and proteins in the neuron. This dynein-LIS1 complex ensures proper positioning of the nucleus during cell division and migration. It also regulates the alignment of chromosomes warranting correct cytoskeleton organization and cell polarity.
- Pathways
LIS1 is deeply involved in neurodevelopmental pathways and the stabilization of the cytoskeleton. The protein is a central player in the Reelin signaling pathway which is essential for layering neurons during cortical development. LIS1 cooperates with other components such as the DISC1 and DCX to ensure proper functioning within these pathways. These interactions are vital for maintaining proper brain structure and neural circuitry.
- Associated diseases and disorders
LIS1 mutations are linked to lissencephaly a disorder characterized by abnormal neuronal migration resulting in a smooth brain structure. This condition stems from disruptions in the mechanisms regulating microtubule dynamics and nuclear positioning during neuronal development. The protein interacts with TUBA1A another protein implicated in lissencephaly affecting the stability and function of microtubules. Disorders such as Miller-Dieker syndrome also relate to abnormalities in LIS1 highlighting its critical role in neurodevelopment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-LIS1 Antibody [S03-2I0] (ab317390), expandable thumbnail](https://content.abcam.com/products/images/lis1-antibody-s03-2i0-ab317390--western-blot-img429474.jpg "Western blot - Anti-LIS1 Antibody [S03-2I0] (ab317390)")
Western blot - Anti-LIS1 Antibody [S03-2I0] (ab317390)
All lanes: Western blot - Anti-LIS1 Antibody [S03-2I0] (ab317390) at 1/1000 dilution
Lane 1: C6 cell lysate
Lane 2: CHO-K1 cell lysate
Predicted band size: 47 kDa
Downloads
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