AB200538

Anti-Listeriolysin (LLO) antibody

Name: Anti-Listeriolysin (LLO) antibody. Rabbit polyclonal (ab200538) | Abcam
Brand: Abcam Limited
SKU: AB200538
Price: 420 USD
Availability: InStock

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(15 Publications)

Rabbit Polyclonal TACY antibody. Suitable for ELISA, WB and reacts with Listeria monocytogenes samples. Cited in 15 publications.

View Alternative Names

hlyA, lisA, lmo0202, hly, Listeriolysin O, LLO, Thiol-activated cytolysin

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Listeria monocytogenes

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Listeria monocytogenes": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Listeriolysin O (LLO), a major virulence factor produced by all pathogenic strains of
L. monocytogenes, has been identified as a candidate antigen for a serological assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2.94% Sodium citrate, 0.61% Tris, 0.05% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cholesterol-dependent pore-forming toxin, which is a major virulence factor required for the escape of bacteria from phagosomal vacuoles and entry into the host cytosol. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Listeriolysin O activates mitogen-activated protein (MAP) kinase activity in host cells, most likely as a result of the permeabilization of the host cell membrane. Also induces a proteasome-independent degradation of UBE2I (the SUMO-conjugating enzyme UBC9) and a proteasome-dependent degradation of some sumoylated proteins. Finally, is necessary and sufficient for spacious Listeria-containing phagosomes (SLAPs) formation, suggesting a role for listeriolysin O in promoting L.monocytogenes replication in vacuoles, leading to persistent infection. Recognized by serum from healthy humans exposed to L.monocytogenes as well from patients who have recovered from listeriosis (PubMed : 9284184).

See full target information hly

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6075 PubMed36241641

2022

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Rupert L Mayer,Rein Verbeke,Caroline Asselman,Ilke Aernout,Adillah Gul,Denzel Eggermont,Katie Boucher,Fabien Thery,Teresa M Maia,Hans Demol,Ralf Gabriels,Lennart Martens,Christophe Bécavin,Stefaan C De Smedt,Bart Vandekerckhove,Ine Lentacker,Francis Impens

British journal of pharmacology 179:3839-3858 PubMed35229287

2022

Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Tingting,Fang Tianqi,Wang Xinyu,Zhang Can,Shen Xue,Deng Xuming,Wang Jianfeng

Nature communications 12:4999 PubMed34404769

2021

Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Joel M J Tan,Monica E Garner,James M Regeimbal,Catherine J Greene,Jorge D Rojas Márquez,Dustin A Ammendolia,Adam R R McCluggage,Taoyingnan Li,Katherine J Wu,Marija Cemma,Philip P Ostrowski,Brian Raught,Michael S Diamond,Sergio Grinstein,Robin M Yates,Darren E Higgins,John H Brumell

NPJ biofilms and microbiomes 7:18 PubMed33558519

2021

Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12-24 h) stage of infection in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xingjian Bai,Dongqi Liu,Luping Xu,Shivendra Tenguria,Rishi Drolia,Nicholas L F Gallina,Abigail D Cox,Ok-Kyung Koo,Arun K Bhunia

Cell reports 30:4027-4040.e7 PubMed32209466

2020

An mRNA-mRNA Interaction Couples Expression of a Virulence Factor and Its Chaperone in Listeria monocytogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitriy Ignatov,Karolis Vaitkevicius,Sylvain Durand,Laty Cahoon,Stefanie S Sandberg,Xijia Liu,Birgitte H Kallipolitis,Patrik Rydén,Nancy Freitag,Ciarán Condon,Jörgen Johansson

The Journal of biological chemistry 294:1202-1217 PubMed30504226

2018

virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Coelho,Lisa Brown,Maria Maryam,Raghav Vij,Daniel F Q Smith,Meagan C Burnet,Jennifer E Kyle,Heino M Heyman,Jasmine Ramirez,Rafael Prados-Rosales,Gregoire Lauvau,Ernesto S Nakayasu,Nathan R Brady,Anne Hamacher-Brady,Isabelle Coppens,Arturo Casadevall

Scientific reports 8:15846 PubMed30367146

2018

Missing elimination via membrane vesicle shedding contributes to the diminished calcium sensitivity of listeriolysin O.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Maurer,Sabrina Hupp,Helena Pillich,Timothy J Mitchell,Trinad Chakraborty,Asparouh I Iliev

PLoS neglected tropical diseases 11:e0005912 PubMed28873442

2017

Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells.

Applications

Species

Unspecified reactive species

Rosângela Salerno-Gonçalves,Hervé Tettelin,David Lou,Stephanie Steiner,Tasmia Rezwanul,Qin Guo,William D Picking,Vishvanath Nene,Marcelo B Sztein

BMC biotechnology 14:77 PubMed25134983

2014

Cytotoxicity of purified listeriolysin O on mouse and human leukocytes and leukaemia cells.

Applications

Species

Unspecified reactive species

Radosław Stachowiak,Marcin Łyżniak,Maja Grabowska,Katarzyna Roeske,Tomasz Jagielski,Jacek Bielecki,Bożena K Budziszewska,Grażyna Hoser,Jerzy Kawiak

Journal of immunology (Baltimore, Md. : 1950) 192:234-44 PubMed24319266

2013

The pore-forming toxin listeriolysin O is degraded by neutrophil metalloproteinase-8 and fails to mediate Listeria monocytogenes intracellular survival in neutrophils.

Applications

Species

Unspecified reactive species

Eusondia Arnett,Stephen Vadia,Colleen C Nackerman,Steve Oghumu,Abhay R Satoskar,Kenneth R McLeish,Silvia M Uriarte,Stephanie Seveau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Listeriolysin (LLO) antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise