AB92274

Anti-Liver Arginase antibody

Name: Anti-Liver Arginase antibody (ab92274) | Abcam
Brand: Abcam Limited
SKU: AB92274
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Goat Polyclonal Liver Arginase antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ARG1 aa 300 to C-terminus.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

2 Images

Unknown

Western blot - Anti-Liver Arginase antibody (AB92274)

All lanes:

Western blot - Anti-Liver Arginase antibody (ab92274) at 0.01 µg/mL

All lanes:

human liver lysate in RIPA buffer at 35 µg

Predicted band size: 35 kDa

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Supplier Data

Western blot - Anti-Liver Arginase antibody (AB92274)

All lanes:

Western blot - Anti-Liver Arginase antibody (ab92274) at 0.03 µg/mL

Lane 1:

pig liver tissue lysate at 35 µg

Lane 2:

mouse liver tissue lysate at 35 µg

Lane 3:

rat liver tissue lysate at 35 µg

Predicted band size: 35 kDa

Observed band size: 35 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human ARG1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05089

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

See full target information ARG1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 51:e13913 PubMed39103233

2024

Down-regulation of CYTL1 attenuates bleomycin-induced pulmonary fibrosis in mice by inhibiting M2 macrophage polarization via the TGF-β/CCN2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Chenxi Liu,Yi Xie,Xiaomei Li

Acta dermato-venereologica 104:adv35401 PubMed38348729

2024

Lesional Infiltration of Eosinophils, Basophils, and M2 Macrophages Expressing Eotaxin-1 and Eotaxin-3 in Granuloma Faciale, but not in Erythema Elevatum Diutinum.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomi Akai,Takashi Hashimoto,Manami Okuzawa,Satoshi Okuno,Takahiro Satoh

NPJ biofilms and microbiomes 9:33 PubMed37280255

2023

Limosilactobacillus mucosae-derived extracellular vesicles modulates macrophage phenotype and orchestrates gut homeostasis in a diarrheal piglet model.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Shuaifei Feng,Zhenyu Wang,Jinhui He,Zeyue Zhang,Huicong Zou,Zhifeng Wu,Xiangdong Liu,Hong Wei,Shiyu Tao

EBioMedicine 91:104555 PubMed37054630

2023

Macrophage CD5L is a target for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Sanchez-Moral,Tony Paul,Clara Martori,Joan Font-Díaz,Lucía Sanjurjo,Gemma Aran,Érica Téllez,Julià Blanco,Jorge Carrillo,Masaoki Ito,Martina Tuttolomondo,Henrik J Ditzel,Caterina Fumagalli,Gustavo Tapia,Julia Sidorova,Helena Masnou,Marco-Antonio Fernández-Sanmartín,Juan-José Lozano,Cristina Vilaplana,Alhelí Rodriguez-Cortés,Carolina Armengol,Annabel F Valledor,Leonor Kremer,Maria-Rosa Sarrias

International journal of medical sciences 20:172-185 PubMed36794154

2023

Adenovirus-IL-10 relieves chronic rejection after mouse heart transplantation by inhibiting miR-155 and activating SOCS5.

Applications

Unspecified application

Species

Unspecified reactive species

Gangcheng Kong,Yuqi Chen,Zongtao Liu,Yixuan Wang,Huadong Li,Chao Guo

eLife 11: PubMed36193890

2022

Telocytes regulate macrophages in periodontal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Anahid A Birjandi,Mohi Ahmed,Yushi Redhead,Jose Villagomez Olea,Paul Sharpe

Cell proliferation 54:e13050 PubMed33960559

2021

Transplantation of tauroursodeoxycholic acid-inducing M2-phenotype macrophages promotes an anti-neuroinflammatory effect and functional recovery after spinal cord injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Gong Ho Han,Seong Jun Kim,Wan-Kyu Ko,Daye Lee,In-Bo Han,Seung Hun Sheen,Je Beom Hong,Seil Sohn

The Journal of investigative dermatology 140:850-859.e3 PubMed31626785

2019

Mechanisms of Itch in Stasis Dermatitis: Significant Role of IL-31 from Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Hashimoto,Christina Dorothy Kursewicz,Rachel Alison Fayne,Sonali Nanda,Serena Maya Shah,Leigh Nattkemper,Hiroo Yokozeki,Gil Yosipovitch

Scientific reports 8:3176 PubMed29453346

2018

Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Jun Kim,Wan-Kyu Ko,Min-Jae Jo,Yoshie Arai,Hyemin Choi,Hemant Kumar,In-Bo Han,Seil Sohn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody

Western blot - Anti-Liver Arginase antibody (AB92274)

Western blot - Anti-Liver Arginase antibody (AB92274)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise