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AB96183

Anti-Liver Arginase antibody

1

(1 Review)

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(32 Publications)

Anti-Liver Arginase antibody (ab96183) is a rabbit polyclonal antibody detecting Liver Arginase in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Over 10 publications
- Trusted since 2010

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

10 Images
Western blot - Anti-Liver Arginase antibody (AB96183)
  • WB

Supplier Data

Western blot - Anti-Liver Arginase antibody (AB96183)

Separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution

All lanes:

Mouse liver extract at 50 µg

Predicted band size: 35 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (AB96183)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (AB96183)

ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody (AB96183)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody (AB96183)

ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

Flow Cytometry - Anti-Liver Arginase antibody (AB96183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Liver Arginase antibody (AB96183)

Flow cytometric analysis of HepG2 cell line labeling ARG1 with ab96183 at 1/50 dilution (red) compared with Isotype Control (black) and an unlabelled control. Dylight 488-conjugated was used as secondary antibody.

Immunoprecipitation - Anti-Liver Arginase antibody (AB96183)
  • IP

Supplier Data

Immunoprecipitation - Anti-Liver Arginase antibody (AB96183)

Arginase 1 was immunoprecipitated from 5μ of HepG2 whole cell lysate with ab96183. Western blot was performed from the immunoprecipitate using ab96183 at 1/600 dilution. EasyBlot anti-Rabbit IgG, was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody (ab96183)

Predicted band size: 35 kDa

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ELISA - Anti-Liver Arginase antibody (AB96183)
  • ELISA

Supplier Data

ELISA - Anti-Liver Arginase antibody (AB96183)

ELISA analysis of HEK-293T whole cell extracts using ab96183 as a detection antibody with concentration of 1 μg/mL.

Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (AB96183)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (AB96183)

Frozen sections of mouse hippocampus tissue stained for Arginase 1 (green) using ab96183 at 1/250 dilution in immunohistochemical analysis. Counterstained with Fluoroshield with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (AB96183)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (AB96183)

Paraffin embedded mouse liver tissue stained for Arginase 1 using ab96183 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Liver Arginase antibody (AB96183)
  • WB

Unknown

Western blot - Anti-Liver Arginase antibody (AB96183)

10% SDS-PAGE

All lanes:

Western blot - Anti-Liver Arginase antibody (ab96183) at 1/3000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Western blot - Anti-Liver Arginase antibody (AB96183)
  • WB

Supplier Data

Western blot - Anti-Liver Arginase antibody (AB96183)

All lanes:

Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution

All lanes:

Rat liver tissue extract at 50 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ELISA, ICC/IF, IHC-Fr, Flow Cyt, IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARG1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P05089

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Liver Arginase antibody (ab96183) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of Liver Arginase?
Anti-Liver Arginase (ab96183) specifically detects a band for Liver Arginase (UniProt: P05089) at a molecular weight of 35kDa.

Trusted by the scientific community
Anti-Liver Arginase (ab96183) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
See full target information ARG1
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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:166 PubMed40684176

2025

Periodontitis-induced neuroinflammation triggers IFITM3-Aβ axis to cause alzheimer's disease-like pathology and cognitive decline.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwenyao Kong,Juanjuan Li,Lu Gao,Yonggang Zhao,Weixian Chen,Xumeng Wang,Songlin Wang,Fu Wang

Journal of orthopaedic translation 52:344-359 PubMed40485847

2025

GPX4 activator enhances neuroprotection and functional recovery in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjie Liu,Yilin Pang,Baoyou Fan,Jiawei Zhang,Shen Liu,Xiaobing Deng,Yun Li,Ying Liu,Xu Zhang,Chenxi Zhao,Xiaoyu Wang,Xudong Wu,Luhua Lai,Shiqing Feng,Wenpeng Liu,Guangzhi Ning,Xue Yao

Journal of nanobiotechnology 23:303 PubMed40259377

2025

PTEN as a prognostic factor for radiotherapy plus immunotherapy response in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Guo,Ming Zhang,Xiaoli Li,Jiashuo Wang

Frontiers in immunology 16:1560996 PubMed40230844

2025

Platelet secretions exert anti-inflammatory effects on neutrophils and uterine epithelia in cattle: a possible role in amplifying the uterine immune network toward pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Samy Yousef,Ihshan Akthar,Dongxue Ma,Shingo Haneda,Kazuya Kusama,Masayuki Shimada,Kazuhiko Imakawa,Akio Miyamoto

Cell biology and toxicology 41:69 PubMed40214820

2025

Based exploration of the diagnostic value of oxidative stress-related key genes in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglan Wang,MingFeng Zhong,Xiaoli Deng,Chen Liu,Yan Tan,Baojiang Qian,MingMei Zhong

Journal of translational medicine 23:60 PubMed39806436

2025

Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shijie Yang,Beibei Yu,Qing Zhang,Yongfeng Zhang,Longhui Fu,Bisheng Zhou,Haining Wu,Jianzhong Li,Shouping Gong

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409882 PubMed39738868

2025

Mild Hyperthermia Accelerates Bone Repair by Dynamically Regulating iNOS/Arg1 Balance in the Early Stage.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhui Zhao,Yiping Luo,Lei Zhang,Yunfeng Chen,Yixing Chen,Xinhui Wu,Aihemaitijiang Aierken,Dilixiati Duolikun,Tianlong Wang,Zifei Zhou,Zhiqing Liu,Longpo Zheng

Neural regeneration research 21:1222-1235 PubMed39589179

2024

Porcine decellularized nerve matrix hydrogel attenuates neuroinflammation after peripheral nerve injury by inhibiting the TLR4/MyD88/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Jianquan Liu,Liuxun Li,Guotian Luo,Xinrong Yuan,Shichao Shen,Yongpeng Shi,Jianlong Wu,Bin Yan,Lei Yang

Cell reports. Medicine 5:101791 PubMed39426375

2024

Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyun Yao,Lingchi Kong,Yi Qiao,David Brand,Juehong Li,Zhiwen Yan,Song Guo Zheng,Yun Qian,Cunyi Fan

Neural regeneration research 20:2611-2623 PubMed39314167

2024

High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mengshi Yang,Miao Bai,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Hao Li,Yu Deng,Hongbin Wu,Xiaojian Xu,Fei Niu,Xinlong Dong,Bin Zhang,Baiyun Liu
View all publications
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