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Rabbit Polyclonal Liver Arginase antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-300.


Images

Flow Cytometry - Anti-Liver Arginase antibody (AB96183), expandable thumbnail
  • Immunoprecipitation - Anti-Liver Arginase antibody (AB96183), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (AB96183), expandable thumbnail
  • Western blot - Anti-Liver Arginase antibody (AB96183), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (AB96183), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ARG1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P05089

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytELISAWBICC/IFIHC-Fr
Human
Tested
Tested
Tested
Tested
Tested
Tested
Expected
Mouse
Tested
Expected
Expected
Expected
Tested
Expected
Tested
Rat
Expected
Expected
Expected
Expected
Tested
Expected
Expected
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/1000

Notes

For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/200

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Rat

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Alternative names

Recommended products

Rabbit Polyclonal Liver Arginase antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-300.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ARG1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

  • Flow Cytometry - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Flow Cytometry - Anti-Liver Arginase antibody (ab96183)

    Flow cytometric analysis of HepG2 cell line labeling ARG1 with ab96183 at 1/50 dilution (red) compared with Isotype Control (black) and an unlabelled control. Dylight 488-conjugated was used as secondary antibody.

  • Immunoprecipitation - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Immunoprecipitation - Anti-Liver Arginase antibody (ab96183)

    Arginase 1 was immunoprecipitated from 5μ of HepG2 whole cell lysate with ab96183. Western blot was performed from the immunoprecipitate using ab96183 at 1/600 dilution. EasyBlot anti-Rabbit IgG, was used as secondary antibody.


    All lanes: Immunoprecipitation - Anti-Liver Arginase antibody (ab96183)

    Predicted band size: 35 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody (ab96183)

    Frozen sections of mouse hippocampus tissue stained for Arginase 1 (green) using ab96183 at 1/250 dilution in immunohistochemical analysis. Counterstained with Fluoroshield with DAPI (blue).

  • Western blot - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody (ab96183)

    Separated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution

    All lanes: Mouse liver extract at 50 µg

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (ab96183)

    Paraffin embedded mouse liver tissue stained for Arginase 1 using ab96183 at 1/500 dilution in immunohistochemical analysis.

  • ELISA - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    ELISA - Anti-Liver Arginase antibody (ab96183)

    ELISA analysis of HEK-293T whole cell extracts using ab96183 as a detection antibody with concentration of 1 μg/mL.

  • Western blot - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody (ab96183)

    All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution

    All lanes: Rat liver tissue extract at 50 µg

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody (ab96183)

    ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry.

  • Western blot - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody (ab96183)

    10% SDS-PAGE

    All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/3000 dilution

    All lanes: Raji whole cell lysate at 30 µg

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody (ab96183), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody (ab96183)

    ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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