Rabbit Polyclonal Liver Arginase antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | IP | Flow Cyt | ELISA | WB | ICC/IF | IHC-Fr | |
---|---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested | Tested | Expected |
Mouse | Tested | Expected | Expected | Expected | Tested | Expected | Tested |
Rat | Expected | Expected | Expected | Expected | Tested | Expected | Expected |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Rabbit | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Select an associated product type
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Rabbit Polyclonal Liver Arginase antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
Up to 12 months
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Flow cytometric analysis of HepG2 cell line labeling ARG1 with ab96183 at 1/50 dilution (red) compared with Isotype Control (black) and an unlabelled control. Dylight 488-conjugated was used as secondary antibody.
Arginase 1 was immunoprecipitated from 5μ of HepG2 whole cell lysate with ab96183. Western blot was performed from the immunoprecipitate using ab96183 at 1/600 dilution. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
All lanes: Immunoprecipitation - Anti-Liver Arginase antibody (ab96183)
Predicted band size: 35 kDa
Frozen sections of mouse hippocampus tissue stained for Arginase 1 (green) using ab96183 at 1/250 dilution in immunohistochemical analysis. Counterstained with Fluoroshield with DAPI (blue).
Separated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution
All lanes: Mouse liver extract at 50 µg
Predicted band size: 35 kDa
Paraffin embedded mouse liver tissue stained for Arginase 1 using ab96183 at 1/500 dilution in immunohistochemical analysis.
ELISA analysis of HEK-293T whole cell extracts using ab96183 as a detection antibody with concentration of 1 μg/mL.
All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution
All lanes: Rat liver tissue extract at 50 µg
Predicted band size: 35 kDa
ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry.
10% SDS-PAGE
All lanes: Western blot - Anti-Liver Arginase antibody (ab96183) at 1/3000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 35 kDa
ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
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