Mouse Monoclonal Liver Arginase antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-150.
Images
![Western blot - Anti-Liver Arginase antibody [ARG1/1125] (AB215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--western-blot-img90516.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Liver Arginase antibody [ARG1/1125] (AB215843)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] (AB215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138536.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] (AB215843)")
![Protein Array - Anti-Liver Arginase antibody [ARG1/1125] (AB215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--protein-array-img141503.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Liver Arginase antibody [ARG1/1125] (AB215843)")
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ARG1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
Abcam Recommends
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Alternative names
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Recommended products
Mouse Monoclonal Liver Arginase antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-150.
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ARG1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
- Clone number
- ARG1/1125
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Liver Arginase antibody [ARG1/1125] (ab215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--western-blot-img90516.jpg "Western blot - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)")
Western blot - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)
All lanes: Western blot - Anti-Liver Arginase antibody [ARG1/1125] (ab215843) at 1 µg/mL
Lane 1: recombinant Human Liver Arginase protein fragment
Lane 2: Human liver lysate
Predicted band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] (ab215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138536.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)
Immunohistochemical analysis of formalix-fixed paraffin-embedded Human hepatocellular carcinoma tissue, labeling Liver Arginase using ab215843 at 4 μg/mL.
![Protein Array - Anti-Liver Arginase antibody [ARG1/1125] (ab215843), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-ab215843--protein-array-img141503.jpg "Protein Array - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)")
Protein Array - Anti-Liver Arginase antibody [ARG1/1125] (ab215843)
ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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