AB212522

Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free

Name: Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) | Abcam
Brand: Abcam Limited
SKU: AB212522

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(8 Publications)

Mouse Monoclonal Liver Arginase antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, Protein Array, ICC/IF and reacts with Human, Recombinant fragment - Human, Recombinant full length protein - Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-150.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling Arginase with ab212522 at 2 μg/mL.

Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

Protein Array

Unknown

Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

This data was produced with ab215843, the same antibody in a different formulation with BSA and Azide.
ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supplier Data

Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

All lanes:

Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) at 0.5 µg/mL

Lane 1:

Recombinant Arginase protein fragment

Lane 2:

Human liver tissue lysate

Predicted band size: 17 kDa,35 kDa

Observed band size: 35-38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ARG1/1125

Isotype

IgG3

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P, Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ARG1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P05089

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Final volume 100 uL.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

See full target information ARG1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell division 20:22 PubMed41039618

2025

FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

One health advances 3:5 PubMed40124736

2025

Arginine as host directed therapy in tuberculosis: insights from modulating arginine metabolism by supplementation and arginase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Qingkui Jiang,Ranjeet Kumar,Yi Zhao,Selvakumar Subbian,Lanbo Shi

Theranostics 13:1325-1341 PubMed36923537

2023

GLSP and GLSP-derived triterpenes attenuate atherosclerosis and aortic calcification by stimulating ABCA1/G1-mediated macrophage cholesterol efflux and inactivating RUNX2-mediated VSMC osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Guobin Zheng,Yun Zhao,Zhenhao Li,Yunqing Hua,Jing Zhang,Yaodong Miao,Yang Guo,Lan Li,Jia Shi,Zhengwei Dong,Shu Yang,Guanwei Fan,Chuanrui Ma

Annals of the New York Academy of Sciences 1514:116-131 PubMed35579934

2022

Negative pressure promotes macrophage M1 polarization after Mycobacterium tuberculosis infection via the lncRNA XIST/microRNA-125b-5p/A20/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Bo Luo,Li-Tao Li,Jian-Cheng Xi,Hong-Tao Liu,Zhen Liu,Long Yu,Pei-Fu Tang

Frontiers in pharmacology 12:658197 PubMed34776939

2021

Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liao,Wanren Yang,Jiecheng Xu,Zhengming Yan,Mingxin Pan,Xiaoping Xu,Shuqin Zhou,Yu Zhu,Jianqiang Lan,Min Zeng,Xu Han,Shao Li,Yang Li,Kangyan Liang,Yi Gao,Qing Peng

BMC infectious diseases 19:1088 PubMed31888505

2019

Identification and characterization of microglia/macrophages in the granuloma microenvironment of encephalic schistosomiasis japonicum.

Applications

Unspecified application

Species

Unspecified reactive species

Zhoubin Tan,Zhuowei Lei,Zhuo Zhang,Huaqiu Zhang,Kai Shu,Feng Hu,Ting Lei

Journal of tissue engineering 10:2041731419889184 PubMed31827757

2019

High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liao,Jieqing Wang,Jiecheng Xu,Fuyu You,Mingxin Pan,Xiaoping Xu,Jun Weng,Xu Han,Shao Li,Yang Li,Kangyan Liang,Qing Peng,Yi Gao

Journal of colloid and interface science 549:50-62 PubMed31015056

2019

Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Sun,Shuxian Yang,Huaihong Cai,Yijin Shu,Qi Han,Baocheng Wang,Zhizhong Li,Libing Zhou,Qingsheng Gao,Zhinan Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise