Mouse Monoclonal Liver Arginase antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, Protein Array, ICC/IF and reacts with Human, Recombinant fragment - Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-150.
Images
![Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--western-blot-img90515.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)")
![Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--protein-array-img141503.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img141008.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (AB212522)")
Publications
Filter by
- Theranostics 13:1325-13412023GLSP and GLSP-derived triterpenes attenuate atherosclerosis and aortic calcification by stimulating ABCA1/G1-mediated macrophage cholesterol efflux and inactivating RUNX2-mediated VSMC osteogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuobin Zheng,Yun Zhao,Zhenhao Li,Yunqing Hua,Jing Zhang,Yaodong Miao,Yang Guo,Lan Li,Jia Shi,Zhengwei Dong,Shu Yang,Guanwei Fan,Chuanrui MaPubMed 36923537
- Frontiers in pharmacology 12:6581972021Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liao,Wanren Yang,Jiecheng Xu,Zhengming Yan,Mingxin Pan,Xiaoping Xu,Shuqin Zhou,Yu Zhu,Jianqiang Lan,Min Zeng,Xu Han,Shao Li,Yang Li,Kangyan Liang,Yi Gao,Qing PengPubMed 34776939
- BMC infectious diseases 19:10882019Identification and characterization of microglia/macrophages in the granuloma microenvironment of encephalic schistosomiasis japonicum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhoubin Tan et. al.PubMed 31888505
- Journal of colloid and interface science 549:50-622019Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuodong Sun et. al.PubMed 31015056
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ARG1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | Protein Array | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected | Expected |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Final volume 100 uL. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Alternative names
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Recommended products
Mouse Monoclonal Liver Arginase antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, Protein Array, ICC/IF and reacts with Human, Recombinant fragment - Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG1 aa 1-150.
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ARG1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
- Carrier free
- Yes
- Clone number
- ARG1/1125
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--western-blot-img90515.png "Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)")
Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
All lanes: Western blot - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) at 0.5 µg/mL
Lane 1: Recombinant Arginase protein fragment
Lane 2: Human liver tissue lysate
Predicted band size: 17 kDa, 35 kDa
Observed band size: 35-38 kDa
![Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--protein-array-img141503.jpg "Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)")
Protein Array - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
This data was produced with Anti-Liver Arginase antibody [ARG1/1125] ab215843, the same antibody in a different formulation with BSA and Azide.
Anti-Liver Arginase antibody [ARG1/1125] ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522), expandable thumbnail](https://content.abcam.com/products/images/liver-arginase-antibody-arg1-1125-bsa-and-azide-free-ab212522--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img141008.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling Arginase with ab212522 at 2 μg/mL.
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