Anti-Liver Arginase antibody [BLR036F] (ab243892)

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARG1 aa 250 to C-terminus.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (AB243892), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05089

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	IP	WB
Human	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Use 20μl/mg lysate.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

See full target information ARG1

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Alternative names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
Clone number: BLR036F
Concentration
Purification notes: Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab243892 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243892 at 1/1000 dilution. Lane 1: ab243892 IP in HepG2 whole cell lysate.
Lane 2: Control IgG in HepG2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Predicted band size: 35 kDa
Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Western blot - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Western blot analysis using ab243892 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW 620 whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
All lanes: Western blot - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (ab243892)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human liver carcinoma tissue labeling liver arginase with ab243892 at 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody. Substrate: Opal. Counterstain: DAPI (blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
Visit troubleshooting

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody [BLR036F]

Related conjugates and formulations

Images

Publications