AB243892

Anti-Liver Arginase antibody [BLR036F]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARG1 aa 250 to C-terminus.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human liver carcinoma tissue labeling liver arginase with ab243892 at 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody. Substrate : Opal. Counterstain : DAPI (blue).

Unknown

Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Unknown

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab243892 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243892 at 1/1000 dilution. Lane 1 : ab243892 IP in HepG2 whole cell lysate.

Lane 2 : Control IgG in HepG2 whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Predicted band size: 35 kDa

false

Unknown

Western blot - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Western blot analysis using ab243892 at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate (50 µg).

Lane 2 : HEK-293T whole cell lysate (50 µg).

Lane 3 : MCF7 whole cell lysate (50 µg).

Lane 4 : HepG2 whole cell lysate (50 µg).

Lane 5 : A549 whole cell lysate (50 µg).

Lane 6 : SW 620 whole cell lysate (50 µg).

Lane 7 : Jurkat whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-Liver Arginase antibody [BLR036F] (ab243892)

Predicted band size: 35 kDa

false

Unconjugated

Anti-Liver Arginase antibody [BLR036F] - BSA free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR036F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05089

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

See full target information ARG1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309471 PubMed38889269

2024

Gut Dysbiosis Drives Inflammatory Bowel Disease Through the CCL4L2-VSIR Axis in Glycogen Storage Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaoli Lan,Yuxin Zhang,Cuiyuan Jin,Huan Chen,Zexiong Su,Jiaxing Wu,Ni Ma,Xiaoyan Zhang,Yiyun Lu,Yongxin Chen,Xiaolu Zeng,Huiqiong Zhang,Guilang Zheng,Yueyu Sun,Chun Wang,Yan Hu,Yifei Wang,Yumei Liu,Zhaoyang Zeng,Liyun Shi,Jun He,Aihua Cao,Yichao Wang,Xu Pan,Gulei Jin,Ying Wang,Xun Jiang,Huiqing Shen,Qing Tang,Xiaoli Xie,Yuan Xiao,Xuemei Zhong,Xuchao Zhang,Liang Zeng,Liping Ye,Jing Xie,Lanlan Geng,Zhiling Li,Xiaohui Wu,Ying Wang,Ren Mao,Shaojun Zhang,Siyuan Huang,Suling Liu,Hanshi Zeng,Wanfu Xu,Sitang Gong,Yuxiong Guo,Min Yang

Frontiers in cellular and infection microbiology 11:693981 PubMed34504806

2021

Transforming Growth Factor-β1 Promotes M1 Alveolar Macrophage Polarization in Acute Lung Injury by Up-Regulating DNMT1 to Mediate the microRNA-124/PELI1/IRF5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqi Wang,Xiaoqing Wang,Hong Zhang,Biao Han,Yuanmei Ye,Mengjie Zhang,Yingbin Wang,Jianjun Xue,Chun'ai Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody [BLR036F]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (AB243892)

Western blot - Anti-Liver Arginase antibody [BLR036F] (AB243892)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise