Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARG1 aa 250 to C-terminus.

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Images

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05089

Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	IP	WB
Human	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information ARG1

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Alternative names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P05089
Clone number: BLR036F
Concentration
Purification notes: Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

ab272083 is the BSA-free version of Anti-Liver Arginase antibody [BLR036F] ab243892.

This product is sold under License from Bethyl Laboratories, Inc.

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6 product images

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with Anti-Liver Arginase antibody [BLR036F] ab243892 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using Anti-Liver Arginase antibody [BLR036F] ab243892 at 1/1000 dilution.
Lane 1: Anti-Liver Arginase antibody [BLR036F] ab243892 IP in HepG2 whole cell lysate.
Lane 2: Control IgG in HepG2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).
All lanes: Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (Anti-Liver Arginase antibody [BLR036F] ab243892)
Predicted band size: 35 kDa
Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using Anti-Liver Arginase antibody [BLR036F] ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).
Western blot - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).
detection: chemiluminescence.
All lanes: Western blot - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 50 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg
Lane 5: A549 (Human lung carcinoma cell line) whole cell lysate at 50 µg
Lane 6: SW 620 whole cell lysate at 50 µg
Lane 7: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 35 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using Anti-Liver Arginase antibody [BLR036F] ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using Anti-Liver Arginase antibody [BLR036F] ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human liver carcinoma tissue labeling liver arginase with Anti-Liver Arginase antibody [BLR036F] ab243892 at 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody. Substrate: Opal. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Liver Arginase antibody [BLR036F] ab243892).

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Anti-Liver Arginase antibody [BLR036F] - BSA free

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