AB272083

Anti-Liver Arginase antibody [BLR036F] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARG1 aa 250 to C-terminus.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human liver carcinoma tissue labeling liver arginase with ab243892 at 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody. Substrate : Opal. Counterstain : DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

Unknown

Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

Unknown

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab243892 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243892 at 1/1000 dilution.

Lane 1 : ab243892 IP in HepG2 whole cell lysate.

Lane 2 : Control IgG in HepG2 whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] (<a href='/en-us/products/primary-antibodies/liver-arginase-antibody-blr036f-ab243892'>ab243892</a>)

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243892).

detection : chemiluminescence.

All lanes:

Western blot - Anti-Liver Arginase antibody [BLR036F] - BSA free (ab272083) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Lane 5:

A549 (Human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 6:

SW 620 whole cell lysate at 50 µg

Lane 7:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 35 kDa

false

Exposure time: 30s

Unconjugated

Anti-Liver Arginase antibody [BLR036F]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR036F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ARG1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05089

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

ab272083 is the BSA-free version of ab243892.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

See full target information ARG1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BioFactors (Oxford, England) 47:865-878 PubMed34339079

2021

CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Guo,Kaimei Zhou,Xiang Zhuang,Junjie Li,Xianglin Shen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody [BLR036F] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Immunocytochemistry - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Immunoprecipitation - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Western blot - Anti-Liver Arginase antibody [BLR036F] - BSA free (AB272083)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise