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Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 14 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (AB233548), expandable thumbnail
  • Western blot - Anti-Liver Arginase antibody [EPR22033-369] (AB233548), expandable thumbnail
  • Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] (AB233548), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Liver Arginase antibody [EPR22033-369] (AB233548), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (AB233548), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Expected
Tested
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/60
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (By similarity). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (PubMed:27043409). Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation; the regulatory outcome seems to be organ specific (PubMed:19360123, PubMed:20483789, PubMed:23552798, PubMed:23637937, PubMed:7537672). In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity (PubMed:19414774, PubMed:23248265).

Alternative names

Arginase-1, Liver-type arginase, Type I arginase, Arg1

Recommended products

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22033-369
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and cytoplasmic staining in rat liver (PMID/ 22298472, 23637937, 21975054) is observed. The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed in RAW 264.7 is consistent with the literature (PMID: 28032600, PMID: 27166374, PMID: 9814991, PMID: 11080091).

    All lanes: Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 37 kDa

    Exposure time: 1s

  • Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Liver Arginase was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab233548 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab233548 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10ug

    Lane 2: ab233548 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab233548 in Mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 seconds

    All lanes: Immunoprecipitation - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Predicted band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse epithelial hepatoma) cells labeling Liver Arginase with ab233548 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and cytoplasmic staining in mouse liver (PMID/ 22298472, 23637937, 21975054). The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27761413). Negative control: kidney (PMID: 21975054)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure Time: 1 second

    All lanes: Western blot - Anti-Liver Arginase antibody [EPR22033-369] (ab233548) at 1/5000 dilution

    Lane 1: Mouse liver lysate at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Rat liver lysate at 20 µg

    Lane 4: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 37 kDa

  • Flow Cytometry (Intracellular) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

    Flow cytometry staining of C57BL/6 Mouse Bone Marrow Cell-derived Macrophages (M1 polarisation) (top) or C57BL/6 Mouse Bone Marrow Cell-derived Macrophages (M2 polarisation) (bottom) with ab233548 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab233548 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106in 100 µl at 5.0 µg/ml (1/472)) for 30min at 22°C. The cells were simultaneously stained with F4/80.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

    Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

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Product protocols

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