Anti-Liver Arginase antibody [EPR6671(B)]

• WB

Lab

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (AB124917)

False colour image of Western blot : Anti-Liver Arginase antibody [EPR6671(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124917 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line ab281603 (knockout cell lysate ab282955). Faint band remaining in KO sample at same molecular weight is likely to be an isoform of arg1. To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

arg1 knockout HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human ARG1 knockout Hep G2 cell line (<a href='/en-us/products/cell-lines/human-arg1-knockout-hep-g2-cell-line-ab281603'>ab281603</a>)

Lanes 3 and 5:

Empty

Lane 4:

Human Liver cell lysate at 5 µg

Lane 6:

A549 cell lysate at 20 µg

Predicted band size: 35 kDa

Observed band size: 36 kDa

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• WB

Unknown

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (AB124917)

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Mouse liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 35 kDa

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• WB

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Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (AB124917)

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution

Lane 1:

Myc-DDK tagged Recombinant Human ARG1 protein (full-length, aa 1 to 322) (34.6 KDa)

Lane 2:

Myc-DDK tagged Recombinant Human ARG2 protein (full-length, aa 1 to 354) (36 KDa）

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 40 kDa

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• WB

CiteAb

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (AB124917)

Liver Arginase western blot using anti-Liver Arginase antibody [EPR6671(B)] ab124917. Publication image and figure legend from Zhao, Y. F., Qiong-Zhang, et al., 2018, Aging Dis, PubMed 30271656.

ab124917 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124917 please see the product overview.

The levels of TNF-α, IL-1β, iNOS/Arg-1 expression, and iNOS/Arg-1 microglia in brain of young and aged mice stimulated with or without LPSMice were dropped into the bilateral nasal cavity with saline or LPS in young or aged mice for 2 months. The levels of TNF-α and IL-1β in brain were assayed by ELISA. A) TNF-α; and B) IL-1β expression in brain was assayed by Western blot. C) Representative bands of iNOS immunostaining. D) Quantitative data of iNOS immunostaining. iNOS/Arg-1 positive microglia in brain was assayed by double immunohistochemistry of iNOS/Arg-1 and CD11b. E) Representative images of iNOS expression on CD11b+ microglia. F) Quantitative data of double positive cells. G) Representative bands of Arg-1 immunostaining. H) Quantitative data of Arg-1 immunostaining. I) Representative images of Arg-1 expression on CD11b+ microglia. J) Quantitative data of double positive cells. Quantitative results are mean ± SEM of 4 mice in each group. *p<0.05, **p<0.01.

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