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Rabbit Recombinant Monoclonal Liver Arginase antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (AB248058), expandable thumbnail
  • Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (AB248058), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Predicted
Not recommended

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Alternative names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

Recommended products

Rabbit Recombinant Monoclonal Liver Arginase antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6671(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248058 is the carrier-free version of Anti-Liver Arginase antibody [EPR6671(B)] ab124917.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (ab248058), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (ab248058)

    False colour image of Western blot: Anti-Liver Arginase antibody [EPR6671(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Liver Arginase antibody [EPR6671(B)] ab124917 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line Human ARG1 knockout Hep G2 cell line ab281603 (knockout cell lysate ab282955). Faint band remaining in KO sample at same molecular weight is likely to be an isoform of arg1. To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (Anti-Liver Arginase antibody [EPR6671(B)] ab124917) at 1/1000 dilution

    Lane 1: Wild-type HepG2 cell lysate at 20 µg

    Lane 2: arg1 knockout HepG2 cell lysate at 20 µg

    Lane 2: Western blot - Human ARG1 knockout Hep G2 cell line (Human ARG1 knockout Hep G2 cell line ab281603)

    Lanes 3 and 5: Empty

    Lane 4: Human Liver cell lysate at 5 µg

    Lane 6: A549 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 35 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (ab248058), expandable thumbnail

    Western blot - Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (ab248058)

    This data was developed using Anti-Liver Arginase antibody [EPR6671(B)] ab124917, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (Anti-Liver Arginase antibody [EPR6671(B)] ab124917) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Mouse liver lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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